Zhang Jing-shu, Chen Bing-qing, Gao Yan-hui, Yang Yan-mei, Wang Xuan-lin, Song Yang, Sun Wen-guang
Department of Nutrition and Food Hygiene, Public Health School of Harbin Medical University, Harbin 150001, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2005 May;39(3):195-8.
To study the effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on critical enzymes of linoleic acid metabolism in stomach granular cell (SGC-7901).
SGC-7901 was treated with c9,t11-CLA by 200, 100, 50 or 25 micromol/L for 24 hours. The effects of c9,t11-CLA on the cell proliferation was measured by monotetrazolium and the expression of Delta6-desaturase, Delta5-desaturase, COX-1, COX-2, 5-LOX mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR).
At a concentration of 200, 100, 50, or 25 micromol/L, c9,t11-CLA suppressed the proliferation of SGC-7901 by 54.3%, 20.5%, 10.5% and 2.93%. The c9,t11-CLA might decrease the expression of COX-2 mRNA, and increase the expression of Delta6-desaturase and COX-1 in SGC-7901, but might not affect Delta5-desaturase and 5-LOX.
The effects of c9,t11-CLA on the COX and Delta6-desaturase might play an important role in mediating the ability of c9,t11-CLA as to inhibiting the proliferation of tumor cells, and the anti-cancer activity by c9,t11-CLA might be associated with the linoleic acid metabolism.
研究共轭亚油酸（c9,t11-CLA）对胃颗粒细胞（SGC-7901）中亚油酸代谢关键酶的影响。
用200、100、50或25微摩尔/升的c9,t11-CLA处理SGC-7901细胞24小时。采用四氮唑盐比色法检测c9,t11-CLA对细胞增殖的影响，采用逆转录聚合酶链反应（RT-PCR）检测Delta6-去饱和酶、Delta5-去饱和酶、COX-1、COX-2、5-脂氧合酶（5-LOX）mRNA的表达。
在200、100、50或25微摩尔/升浓度下，c9,t11-CLA对SGC-7901细胞增殖的抑制率分别为54.3%、20.5%、10.5%和2.93%。c9,t11-CLA可能降低SGC-7901细胞中COX-2 mRNA的表达，增加Delta6-去饱和酶和COX-1的表达，但对Delta5-去饱和酶和5-LOX无影响。
c9,t11-CLA对COX和Delta6-去饱和酶的影响可能在介导其抑制肿瘤细胞增殖能力中起重要作用，c9,t11-CLA的抗癌活性可能与亚油酸代谢有关。
