Yang Yanmei, Chen Bingqing, Xue Yingben, Zheng Yumei
Public Health School of Harbin Medical University, Harbin 150001, China.
Wei Sheng Yan Jiu. 2003 Mar;32(2):117-9.
In order to study the effects of c9, t11-conjugated linoleic acid on the invasion of human gastric carcinoma cell line(SGC-7901) and explore its possible mechanism, the five levels of CLA in medium were designed as 0, 25, 50, 100 and 200 mmol/L respectively. After SGC-7901 were treated with c9, t11-CLA for 24 hours, the reconstituted basement membrane invasion assay was used to evaluate invasive ability of cancer cells. The type IV collagenase was assessed by PAGE substrate zymography and the expression of TIMP-1 and TIMP-2 mRNA was measured by RT-PCR. The result displayed that c9, t11-CLA could inhibit the reconstituted basement memebrane invasion, reduce type IV collagenase activities in the serum-free supernatant of SGC-7901 cells and induce TIMP-1 and TIMP-2 mRNA expression in SGC-7901 cell line. Therefore, it is suggested that CLA could inhibit the reconstituted basement membrane invasion of SGC-7901 cells and the anti-invasion action of c9, t11-CLA might be associated with the reduction of type IV collagenase activity in tumor cell culture supernatant and induction of tumor cell TIMP-1 and TIMP-2 mRNA expression.
为研究c9，t11-共轭亚油酸对人胃癌细胞系（SGC-7901）侵袭的影响并探讨其可能机制，将培养基中CLA的五个水平分别设计为0、25、50、100和200 mmol/L。用c9，t11-CLA处理SGC-7901 24小时后，采用重组基底膜侵袭试验评估癌细胞的侵袭能力。通过PAGE底物酶谱法评估IV型胶原酶，通过RT-PCR检测TIMP-1和TIMP-2 mRNA的表达。结果显示，c9，t11-CLA可抑制重组基底膜侵袭，降低SGC-7901细胞无血清上清液中IV型胶原酶活性，并诱导SGC-7901细胞系中TIMP-1和TIMP-2 mRNA表达。因此，提示CLA可抑制SGC-7901细胞的重组基底膜侵袭，c9，t11-CLA的抗侵袭作用可能与肿瘤细胞培养上清液中IV型胶原酶活性降低及肿瘤细胞TIMP-1和TIMP-2 mRNA表达诱导有关。
