原发性开角型青光眼房水中透明质酸的浓度。

Concentration of hyaluronic acid in primary open-angle glaucoma aqueous humor.

作者信息

Navajas Eduardo Vitor, Martins João Roberto Maciel, Melo Luiz Alberto S, Saraiva Vinícius Silveira, Dietrich Carl Peter, Nader Helena Bonciani, Belfort Rubens

机构信息

Department of Ophthalmology, Federal University of São Paulo, Rua Botucatu 820, São Paulo, SP 04023-062, Brazil.

出版信息

Exp Eye Res. 2005 Jun;80(6):853-7. doi: 10.1016/j.exer.2004.12.016. Epub 2005 Jan 26.

DOI:10.1016/j.exer.2004.12.016

PMID:15939042

Abstract

We compared the concentration of hyaluronic acid in the aqueous humor of primary open-angle glaucoma (POAG) patients and non-glaucomatous patients. Aqueous humor samples were obtained from 22 patients just before trabeculectomy for clinically uncontrolled POAG (POAG group). Aqueous humor (0.1 mL) was aspirated by inserting a 26-gauge needle into the anterior chamber. The same procedure was performed for 22 non-glaucomatous patients just before cataract surgery (control group). Immediately after collection, the aqueous humor was stored at -20 degrees C. The concentration of hyaluronic acid was determined by a sensitive, noncompetitive and nonisotopic fluoroassay. The median (range) concentrations of hyaluronic acid of the POAG and control groups were 298.4 microg L(-1) (99.0-743.7 microg L(-1)) and 545.1 microg L(-1) (145.0-2366.0 microg L(-1)), respectively. The difference in concentrations of hyaluronic acid between the groups was statistically significant (P<0.001). In conclusion, the concentration of hyaluronic acid in the aqueous humor in POAG patients is lower than in non-glaucomatous patients. Further studies are necessary to determine the role of hyaluronic acid in the pathophysiology of POAG.

摘要

我们比较了原发性开角型青光眼（POAG）患者与非青光眼患者房水中透明质酸的浓度。从22例临床控制不佳的POAG患者（POAG组）小梁切除术前获取房水样本。通过将26号针头插入前房抽取0.1 mL房水。对22例非青光眼患者在白内障手术前进行相同操作（对照组）。采集后立即将房水储存在-20℃。采用灵敏、非竞争性、非同位素荧光分析法测定透明质酸浓度。POAG组和对照组透明质酸的中位（范围）浓度分别为298.4 μg L⁻¹（99.0 - 743.7 μg L⁻¹）和545.1 μg L⁻¹（145.0 - 2366.0 μg L⁻¹）。两组间透明质酸浓度差异具有统计学意义（P<0.001）。总之，POAG患者房水中透明质酸浓度低于非青光眼患者。有必要进一步研究以确定透明质酸在POAG病理生理学中的作用。