Chan L N, Wacholtz M, Sha'afi R I
Membr Biochem. 1978;1(1-2):159-76. doi: 10.3109/09687687809064164.
We found considerable differences in the pattern of membrane proteins as well as in the relative amounts of individual components in isolated chick red blood cell membranes during the course of embryonic development. Of special interest in the increase in the relative amounts of two major polypeptides, band 3 and 3.1 (MW 100,000 daltons) with increasing age of the cells. With respect to functional studies, we found that the magnitude of sulfate influx decreases with increasing age of the embryo. Furthermore, the activity of ouabain-sensitive ATPase increases with increasing age (2.5-day embryo to adult). In addition, both the basal and the fluoride-stimulated adenylate cyclase activities decrease as the embryo age increases, whereas the enzyme sensitivity to epinephrine increases with increasing age of the embryo.
我们发现在胚胎发育过程中，分离出的鸡红细胞膜上的膜蛋白模式以及各个成分的相对含量存在显著差异。特别值得关注的是，随着细胞年龄的增长，两种主要多肽带3和带3.1（分子量100,000道尔顿）的相对含量增加。关于功能研究，我们发现随着胚胎年龄的增长，硫酸盐内流的幅度降低。此外，哇巴因敏感的ATP酶活性随着年龄增长而增加（从2.5天的胚胎到成年）。另外，随着胚胎年龄的增加，基础和氟化物刺激的腺苷酸环化酶活性均降低，而该酶对肾上腺素的敏感性随着胚胎年龄的增加而增加。
