Fu Yong Bi, Peterson Gregory W, Williams David, Richards Ken W, Fetch Jennifer Mitchell
Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2, SK, Canada.
Theor Appl Genet. 2005 Aug;111(3):530-9. doi: 10.1007/s00122-005-2044-2. Epub 2005 Jun 7.
Many core collections have been developed from large collections of crop germplasm, but most of these have not been characterized, particularly using molecular techniques, for germplasm management and utilization. We have attempted to characterize a structured sample representing a world collection of 11,622 cultivated hexaploid oat accessions in the hope of understanding the genetic structure of the world collection. The amplified fragment length polymorphism (AFLP) technique was applied to screen 670 accessions representing 79 countries and one group of uncertain origin. For each accession, 170 AFLP polymorphic bands detected by five AFLP primer pairs were scored. Analyses of the AFLP data showed the effectiveness of the stratified sampling applied in capturing country-wise AFLP variation. The frequencies of polymorphic bands ranged from 0.11 to 0.99, with an average of 0.72. The majority (89.9%) of the AFLP variation resided within accessions of each country, and only 6.2% of the AFLP differences existed among accessions of major geographic regions. Accessions from the Mediterranean region were the most distinct, while those from Russia and the USA were the most diverse. The two distinct groups that were observed were separated largely on the basis of common oat and red oat. Red oat was genetically more diverse than its common and hull-less counterparts, and hull-less oat was more related to common oat than red oat. Landrace and non-landrace accessions displayed similar AFLP variation patterns. These patterns are significant for understanding the domestication of cultivated oat and are useful in classifying the intraspecific diversity of oat germplasm, developing specific core subsets of the oat collection, and exploring new sources of genes for oat improvement.
许多核心种质库是从大量作物种质资源中构建而成的，但其中大多数尚未针对种质管理和利用进行特征鉴定，特别是未采用分子技术。我们试图对一个结构化样本进行特征鉴定，该样本代表了包含11622份栽培六倍体燕麦种质的全球种质库，以期了解全球种质库的遗传结构。应用扩增片段长度多态性（AFLP）技术对代表79个国家和一组来源不明的670份种质进行筛选。对于每份种质，对由五对AFLP引物对检测到的170条AFLP多态性条带进行评分。AFLP数据分析表明，分层抽样在捕获各国AFLP变异方面是有效的。多态性条带的频率范围为0.11至0.99，平均为0.72。大部分（89.9%）的AFLP变异存在于每个国家的种质内，主要地理区域的种质间仅存在6.2%的AFLP差异。地中海地区的种质最为独特，而俄罗斯和美国的种质最为多样。观察到的两个不同组主要是根据普通燕麦和红燕麦区分开来的。红燕麦在遗传上比其普通燕麦和裸燕麦对应种质更加多样，并且裸燕麦与普通燕麦的亲缘关系比与红燕麦的更近。地方品种和非地方品种的种质表现出相似的AFLP变异模式。这些模式对于理解栽培燕麦的驯化具有重要意义，并且有助于对燕麦种质的种内多样性进行分类、开发燕麦种质库的特定核心子集以及探索改良燕麦的新基因来源。
