Schoen D J, Brown A H
Department of Biology, McGill University, Montreal, PQ, Canada.
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10623-7. doi: 10.1073/pnas.90.22.10623.
Wild crop relatives are an important source of genetic variation for improving domesticated species. Given limited resources, methods for maximizing the genetic diversity of collections of wild relatives are needed to help spread protection over a larger number of populations and species. Simulations were conducted to investigate the optimal strategy of sampling materials from populations of wild relatives, with the objective of maximizing the number of alleles (allelic richness) in collections of fixed size. Two methods, based on assessing populations for variation at marker loci (e.g., allozymes, restriction fragment length polymorphisms), were developed and compared with several methods that are not dependent on markers. Marker-assisted methods yielded higher overall allelic richness in the simulated collections, and they were particularly effective in conserving geographically localized alleles, the class of alleles that is most subject to loss.
野生作物近缘种是改良驯化物种的重要遗传变异来源。鉴于资源有限，需要采用方法来最大化野生近缘种收集品的遗传多样性，以帮助将保护范围扩大到更多的种群和物种。进行了模拟研究，以调查从野生近缘种种群中采样材料的最佳策略，目的是在固定大小的收集品中最大化等位基因数量（等位基因丰富度）。开发了两种基于评估标记位点（如同工酶、限制性片段长度多态性）变异的方法，并与几种不依赖标记的方法进行了比较。在模拟收集品中，标记辅助方法产生了更高的总体等位基因丰富度，并且在保护地理上局部化的等位基因方面特别有效，这类等位基因最容易丢失。
