de Mello Marcela Freire Vallim, Monteiro Ana Beatriz Soares, Fonseca Eliene Carvalho, Pissinatti Alcides, Ferreira Ana Maria Reis
Postgraduate Course in Experimental Pathology, Department of Pathology, Universidade Federal Fluminense, Rio de Janeiro, Brasil.
Am J Primatol. 2005 Jun;66(2):111-8. doi: 10.1002/ajp.20131.
The aim of this study was to identify the presence of Helicobacter sp. in the gastric mucosa of captive marmosets (Callithrix sp.). Histologic specimens from the fundic, corpus, and antral gastric regions of six Callithrix jacchus, 12 C. kuhli, and 12 C. geoffroyi specimens were evaluated. The sections were stained with hematoxylin-eosin (H&E) and the Warthin-Starry silver impregnation method, and immunostained with rabbit anti-H. pylori polyclonal antibody. Helicobacter-like organisms (HLOs) and coccoid forms were present in silver-stained sections from 29 stomachs, whereas immunohistochemistry (IHC) tests revealed bacterial aggregates in 15 stomachs. No statistical difference relative to the presence of Helicobacter sp. was found among the gastric regions or marmoset species. Gastric lesions were found in the groups of marmosets that had positive and negative IHC results, but no correlation between inflammation and Helicobacter sp. infection was established. These findings demonstrate that marmosets are susceptible to naturally-occurring Helicobacter sp. infection, and open the way to the development of comparative studies on Helicobacter sp. infection in humans.
本研究的目的是确定圈养狨猴（绢毛猴属）胃黏膜中幽门螺杆菌属的存在情况。对6只普通狨猴、12只库氏狨猴和12只杰氏狨猴的胃底、胃体和胃窦区域的组织学标本进行了评估。切片用苏木精-伊红（H&E）染色和沃辛-斯塔瑞银浸染法染色，并用兔抗幽门螺杆菌多克隆抗体进行免疫染色。在29个胃的银染切片中存在幽门螺杆菌样生物体（HLOs）和球形体，而免疫组织化学（IHC）检测在15个胃中发现了细菌聚集体。在胃区域或狨猴物种之间，相对于幽门螺杆菌属的存在未发现统计学差异。在免疫组织化学结果为阳性和阴性的狨猴组中均发现了胃部病变，但未确定炎症与幽门螺杆菌属感染之间的相关性。这些发现表明，狨猴易受自然发生的幽门螺杆菌属感染，并为开展关于人类幽门螺杆菌属感染的比较研究开辟了道路。
