Kim Deok-Ho, Sun Yu, Yun Seok, Lee Sang Ho, Kim Byungkyu
Microsystems Research Center, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul 130-650, Republic of Korea.
J Biomech. 2005 Jun;38(6):1359-3. doi: 10.1016/j.jbiomech.2004.07.014.
The zebrafish is a model organism for addressing questions of vertebrate embryo development. In this paper, the softening phenomenon of the chorion envelope of zebrafish embryos at different developmental stages was mechanically quantitated by using a microrobotic force sensing system. The microrobotic system integrates a piezoelectric cellular force sensor to measure the required forces for penetrating the chorion envelope. Magnitude of penetration forces was found to decrease as an embryo develops. The results mechanically quantitate "chorion softening" in zebrafish embryos due to protease activities subtly modifying the chorion structure, providing an understanding of zebrafish embryo development.
斑马鱼是用于解决脊椎动物胚胎发育问题的模式生物。在本文中，使用微机器人力传感系统对不同发育阶段斑马鱼胚胎的绒毛膜包膜软化现象进行了力学定量分析。该微机器人系统集成了一个压电细胞力传感器，用于测量穿透绒毛膜包膜所需的力。研究发现，随着胚胎发育，穿透力的大小会降低。这些结果从力学角度定量分析了由于蛋白酶活性对绒毛膜结构的微妙修饰而导致的斑马鱼胚胎“绒毛膜软化”，有助于理解斑马鱼胚胎的发育过程。
