Chang Zhao-rui, Yang Ren-quan, Wang Yan-bin, Ren Li-li, Wang Min, Yang Yao-wu, Guo Li, Chao Yan-gong, Qu Cheng-yi, Wang Jian-wei, Hong Tao
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Mar;26(3):201-3.
To examine the RNA of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) in the blood and excretion of convalescent patient with SARS for prevention and treatment of the disease.
A total of 276 samples, including plasma, urine, feces and sputum, obtained from 23 convalescent patients with SARS were studied at 3 time-points at least 21 days after the onset of symptoms. RNA was extracted and nested reverse transcription-polymerase chain reaction (RT-PCR) was carried out using SARS-CoV specific primers.
Among the 276 samples, SARS-CoV RNA was detected in 6 cases (38.8%) by nested RT-PCR. The positive rates of SARS-CoV RNA was 5.8% in feces and 2.9% in sputum samples but SARS-CoV RNA was not detectable in plasma and urine of all the cases.
The existence of SARS-CoV RNA in the excretion of some convalescent patients with SARS showed that the excretion from these patients should be carefully treated whilthe re-transmission of SARS by which, should be further studied.
检测严重急性呼吸综合征相关冠状病毒（SARS-CoV）康复患者血液及排泄物中的RNA，以用于该疾病的防治。
对23例SARS康复患者在症状出现至少21天后的3个时间点采集的共276份样本（包括血浆、尿液、粪便和痰液）进行研究。提取RNA，并使用SARS-CoV特异性引物进行巢式逆转录-聚合酶链反应（RT-PCR）。
在276份样本中，通过巢式RT-PCR在6例（38.8%）中检测到SARS-CoV RNA。粪便样本中SARS-CoV RNA阳性率为5.8%，痰液样本中为2.9%，但所有病例的血浆和尿液中均未检测到SARS-CoV RNA。
部分SARS康复患者排泄物中存在SARS-CoV RNA，表明应对这些患者的排泄物进行谨慎处理，同时SARS由此再次传播的情况仍需进一步研究。
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