[应用巢式逆转录聚合酶链反应检测严重急性呼吸综合征患者粪便标本中冠状病毒RNA及其临床意义]

[Detection of SARS-CoV RNA in stool samples of SARS patients by nest RT-PCR and its clinical value].

作者信息

Ren Yi, Ding Hui-guo, Wu Qing-fa, Chen Wei-jun, Chen Dong, Bao Zhi-ying, Yang Ling, Zhao Chun-hui, Wang Jian

机构信息

Department of Infectious Diseases, Beijing You'an Hospital, Capital University of Medicine Science, Beijing 100054, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Jun;25(3):368-71.

PMID:12905760

Abstract

OBJECTIVE

To investigate of severe acute respiratory syndrome (SARS) convalescent stool shedding by RT-PCR.

METHODS

One hundred and three stool samples from 46 SARS patients were collected on May 16th, 20th, and 23rd, 2003. For each sample, RNA was extracted using commercial kit and 7 Nest RT-PCR using a 14-pair different SARS-associated coronavirus (SARS-CoV) special primers were carried out simultaneously.

RESULTS

Among these 46 SARS patients, 17 cases (37.0%) were stool SARS-CoV RT-PCR negative, and 29 cases (63.0%) were SARS-CoV RT-PCR positive. The duration of positive cases lasted (31.76 +/- 10.78) d (12-64 d). The longest stool shedding case in this study lasted 64 days. Two serial stool samples and for each sample 2 RT-PCR tests using different primers were positive in this case.

CONCLUSIONS

Our study observed longest stool shedding of SARS patients to be 64 days after initial onset of SARS. The average stool shedding was 32 days. Hence it is important to think highly of SARS convalescent patient stool sterilization.

摘要

目的

通过逆转录聚合酶链反应（RT-PCR）研究严重急性呼吸综合征（SARS）康复期患者粪便排毒情况。

方法

于2003年5月16日、20日和23日收集46例SARS患者的103份粪便样本。每份样本使用商业试剂盒提取RNA，并同时使用14对不同的严重急性呼吸综合征相关冠状病毒（SARS-CoV）特异性引物进行7轮巢式RT-PCR。

结果

在这46例SARS患者中，17例（37.0%）粪便SARS-CoV RT-PCR检测为阴性，29例（63.0%）为阳性。阳性病例的排毒持续时间为（31.76±10.78）天（12 - 64天）。本研究中粪便排毒时间最长的病例持续了64天。该病例的两份连续粪便样本，每份样本使用不同引物进行2次RT-PCR检测均为阳性。