McDonald C P, Colvin J, Robbins S, Barbara J A J
National Blood Service, North London, London, UK.
Transfus Med. 2005 Jun;15(3):175-83. doi: 10.1111/j.1365-3148.2005.00569.x.
Blood services worldwide are now striving to reduce the risk of transmission of bacteria by transfusion. The BacT/ALERT microbial detection system (bioMerieux, Basingstoke, Hants, UK) is currently regarded as the 'gold standard' for bacterial screening of platelet concentrates. The BacT/ALERT is a culture system and will not generate an 'instant' (within 2 h) determination. We report on the Scansystem (Hemosystem, Marseille, France), a solid-phase fluorescent cytometric technique, which enables the rapid detection of bacteria (within 90 min) in platelet concentrates. The study was performed in two parts - one involving the routine screening of platelet concentrates and the other determining the sensitivity of the system. In both arms of the study, the BacT/ALERT was used for comparative purposes. In total, 900 platelet concentrates were screened (63 apheresis and 837 buffy coat pooled). No bacteria were detected in any of the platelet concentrates tested by means of either the Scansystem or the BacT/ALERT. The sensitivity of the Scansystem was in the order of 10(3) cfu mL(-1). Escherichia coli and Staphylococcus aureus were detected by using the Scansystem at 1 cfu mL(-1). The BacT/ALERT detected all organisms tested (n = 6) at 1 cfu mL(-1). The Scansystem offers a sensitive alternative technology to bacterial culture, with the benefit of a rapid test time.
全球的血液服务机构目前都在努力降低输血传播细菌的风险。BacT/ALERT微生物检测系统（法国生物梅里埃公司，英国汉普郡贝辛斯托克）目前被视为血小板浓缩物细菌筛查的“金标准”。BacT/ALERT是一种培养系统，无法进行“即时”（2小时内）检测。我们报告了Scansystem（法国血液系统公司，马赛），一种固相荧光细胞计数技术，它能够快速检测血小板浓缩物中的细菌（90分钟内）。该研究分两部分进行——一部分涉及血小板浓缩物的常规筛查，另一部分确定该系统的灵敏度。在研究的两个环节中，BacT/ALERT都用作对照。总共筛查了900份血小板浓缩物（63份单采血小板和837份混合浓缩红细胞）。通过Scansystem或BacT/ALERT检测的任何血小板浓缩物中均未检测到细菌。Scansystem的灵敏度约为10(3) cfu mL(-1)。使用Scansystem在1 cfu mL(-1)时检测到了大肠杆菌和金黄色葡萄球菌。BacT/ALERT在1 cfu mL(-1)时检测到了所有测试的微生物（n = 6）。Scansystem为细菌培养提供了一种灵敏的替代技术，具有检测时间短的优点。
