Kamalakkannan Narasimhanaidu, Rukkumani Rajagopalan, Varma Penumathsa Suresh, Viswanathan Periyasamy, Rajasekharan Kallikat Narayanan, Menon Venugopal Padmanabhan
Department of Biochemistry, Faculty of Science, Rajah Muthiah Medical College, Annamalai University, Annamalainagar-608002, Tamil Nadu, India.
Basic Clin Pharmacol Toxicol. 2005 Jul;97(1):15-21. doi: 10.1111/j.1742-7843.2005.pto_97103.x.
We have evaluated the comparative effect of curcumin (diferuloyl methane) and its analogue [bis-1,7-(2-hydroxyphenyl)-hepta-1,6-diene-3,5-dione] (BDMC-A) on carbon tetrachloride-induced hepatotoxicity in rats. Administration of carbon tetrachloride (3 ml/kg/week) for three months significantly (P<0.05) increased the levels of marker enzymes such as aspartate transaminase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT). The levels of plasma thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides were also significantly (P<0.05) increased. We have observed a significant (P<0.05) decrease in the levels of plasma reduced glutathione (GSH), vitamin C and vitamin E. There was a significant (P<0.05) increase in the levels of TBARS and hydroperoxides in liver and kidney and a significant (P<0.05) decrease in the activities of enzymic antioxidants- superoxide dismutase (SOD), catalase and GSH peroxidase along with GSH in CCl(4)-treated rats. Oral administration of curcumin and BDMC-A to CCl(4)-induced rats for a period of three months significantly (P<0.05) decreased the levels of marker enzymes, plasma TBARS and hydroperoxides and increased the levels of plasma and tissue antioxidants. Histopathological studies of liver also showed protective effect of curcumin and BDMC-A. We have observed thickening of blood vessels and microvesicular fatty changes around the portal triad in CCl(4)-treated rat liver. Treatment with curcumin showed only mild sinusoidal dilatation while with BDMC-A there was only mild portal inflammation. The effect exerted by BDMC-A was found to be more promising than curcumin.
我们评估了姜黄素（二阿魏酰甲烷）及其类似物[双-1,7-（2-羟苯基）-庚-1,6-二烯-3,5-二酮]（BDMC-A）对四氯化碳诱导的大鼠肝毒性的比较作用。连续三个月每周给予大鼠四氯化碳（3 ml/kg）显著（P<0.05）提高了标志物酶如天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）和γ-谷氨酰转移酶（GGT）的水平。血浆硫代巴比妥酸反应性物质（TBARS）和脂质氢过氧化物水平也显著（P<0.05）升高。我们观察到血浆还原型谷胱甘肽（GSH）、维生素C和维生素E水平显著（P<0.05）降低。在四氯化碳处理的大鼠中，肝脏和肾脏中TBARS和氢过氧化物水平显著（P<0.05）升高，酶促抗氧化剂超氧化物歧化酶（SOD）、过氧化氢酶和GSH过氧化物酶以及GSH的活性显著（P<0.05）降低。对四氯化碳诱导的大鼠口服姜黄素和BDMC-A三个月显著（P<0.05）降低了标志物酶、血浆TBARS和氢过氧化物水平，并提高了血浆和组织抗氧化剂水平。肝脏的组织病理学研究也显示了姜黄素和BDMC-A的保护作用。我们观察到四氯化碳处理的大鼠肝脏门三联周围血管增厚和微泡性脂肪变性。姜黄素处理仅显示轻度的窦状扩张，而BDMC-A处理仅显示轻度的门脉炎症。发现BDMC-A的作用比姜黄素更有前景。
