Begum Rayhana, Papia Sonia Akther, Begum Mst Marium, Wang Hongbin, Karim Rubaba, Sultana Rebeka, Das Priyanka Rani, Begum Taslima, Islam Md Ragibul, Manwar Nargis, Rahman Md Sohanur
Department of Pharmacy, Primeasia University, Dhaka, Bangladesh.
Department of Pharmacy, East West University, Aftabnagar, Dhaka-1212, Bangladesh.
Adv Pharmacol Pharm Sci. 2022 Feb 27;2022:3169500. doi: 10.1155/2022/3169500. eCollection 2022.
Polyherbal formulations (PLFs) have been widely used for liver protection, treatment for hepatic dysfunction, and regeneration. They can also enhance appetite and protect the gastrointestinal tract from injury. In spite of the prevalent use, there is a need of scientific evidence on their effectiveness and safety. The objective of the present study was to assess the hepatoprotective effect of polyherbal formulations (commercially available in Bangladesh namely Heptaliv, Holyliv, Icturn, and J-deenar) in CCl-induced hepatotoxicity in mice.
In this study, Swiss albino mice were treated for 7 days with distilled water or PLFs (2.6 and 5.2 ml/kg body weight/day, per os.) followed by single subcutaneous injection of CCl (1 ml/kg body weight, diluted with olive oil in 1 : 1 ratio) on day 8. Twenty-four hours after CCl administration, the mice were monitored for the effects of PLFs on liver morphology, biochemical parameters including serum aspartate transaminase (AST), serum alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin. Phenobarbitone-induced sleeping time and histopathology changes in liver tissues were also monitored.
CCl administration caused significant hepatotoxicity as evidenced by marked elevation in AST, ALT, ALP, and total bilirubin. Phenobarbitone-induced sleeping time and infiltration of inflammatory cells and centrizonal necrosis on histological examination of liver demonstrated hepatic injury after CCl administration. However, the administration of Icturn and J-deenar polyherbal formulations at the higher dose significantly decreased the levels of AST, ALT, ALP, and total bilirubin. Moreover, pentobarbitone-induced sleeping time and histopathological analysis also revealed significant improvement as result of treatment with formulations Icturn and J-deenar.
Our results confirmed that polyherbal formulations (Icturn and J-deenar) can significantly prevent CCl-induced hepatotoxicity in mice, demonstrating their protective effect for liver.
多草药配方（PLFs）已被广泛用于肝脏保护、肝功能障碍治疗及肝脏再生。它们还能增进食欲并保护胃肠道免受损伤。尽管其应用广泛，但仍需要关于其有效性和安全性的科学证据。本研究的目的是评估多草药配方（在孟加拉国可商购的Heptaliv、Holyliv、Icturn和J-deenar）对四氯化碳诱导的小鼠肝毒性的肝保护作用。
在本研究中，瑞士白化小鼠用蒸馏水或PLFs（2.6和5.2毫升/千克体重/天，经口给药）处理7天，随后在第8天单次皮下注射四氯化碳（1毫升/千克体重，与橄榄油按1∶1比例稀释）。四氯化碳给药24小时后，监测小鼠肝脏形态、生化参数（包括血清天冬氨酸转氨酶（AST）、血清丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和总胆红素）方面PLFs的作用。还监测了苯巴比妥诱导的睡眠时间及肝组织的组织病理学变化。
四氯化碳给药导致显著的肝毒性，表现为AST、ALT、ALP和总胆红素显著升高。苯巴比妥诱导的睡眠时间以及肝脏组织学检查显示的炎性细胞浸润和中央区坏死表明四氯化碳给药后出现了肝损伤。然而，高剂量的Icturn和J-deenar多草药配方给药显著降低了AST、ALT、ALP和总胆红素水平。此外，戊巴比妥诱导的睡眠时间及组织病理学分析也显示Icturn和J-deenar配方治疗后有显著改善。
我们的结果证实，多草药配方（Icturn和J-deenar）可显著预防四氯化碳诱导的小鼠肝毒性，证明了它们对肝脏的保护作用。
