新鲜冰冻血浆中可溶性A物质与ABO血型、分泌型基因型及Lewis表型的关系

Soluble type A substance in fresh-frozen plasma as a function of ABO and Secretor genotypes and Lewis phenotype.

作者信息

Achermann F J, Julmy F, Gilliver L G, Carrel T P, Nydegger U E

机构信息

University Clinic of Cardiovascular Surgery, HGEK Inselspital, CH-3010 Bern, Switzerland.

出版信息

Transfus Apher Sci. 2005 Jun;32(3):255-62. doi: 10.1016/j.transci.2004.05.007.

DOI:10.1016/j.transci.2004.05.007

PMID:15944111

Abstract

Soluble ABO blood group substance (SAS) in fresh-frozen plasma (FFP) and its cognate alloantibody titer reduction capacity (TRC) are not considered when prescribing this product for plasma exchange (PEX) therapy of ABO incompatible transplant recipients. SAS was quantified in 250 single FFPs using ELISA. Total and IgG class-specific anti-A TRCs of FFPs were measured using a microhemagglutination inhibition assay. SAS level depended not only on the A subtype (p < 0.0001) and the Secretor status (p < 0.0001), but also on the expression of ALe(b) in A1 secretors (p < 0.0001). The variation was as great as 137.6 arbitrary units (aU) for 14 A1 Le(a-b-) secretors and 1.2 aU for 6 A2 non-secretors. Homozygous expression of the A1, A2 and Secretor alleles did not increase SAS levels. Only total anti-A TRC, but not IgG class-specific TRC depended on the detected SAS level (r = 0.566, p = 0.0003).

摘要

在为ABO血型不相容的移植受者开具用于血浆置换（PEX）治疗的新鲜冰冻血浆（FFP）产品时，未考虑新鲜冰冻血浆中的可溶性ABO血型物质（SAS）及其同源同种异体抗体效价降低能力（TRC）。使用酶联免疫吸附测定法（ELISA）对250份单一FFP中的SAS进行了定量。使用微血凝抑制试验测量FFP的总抗A TRC和IgG类特异性抗A TRC。SAS水平不仅取决于A亚型（p<0.0001）和分泌状态（p<0.0001），还取决于A1分泌型中ALe(b)的表达（p<0.0001）。14名A1 Le(a-b-)分泌型的差异高达137.6任意单位（aU），6名A2非分泌型的差异为1.2 aU。A1、A2和分泌型等位基因的纯合表达并未增加SAS水平。只有总抗A TRC，而不是IgG类特异性TRC取决于检测到的SAS水平（r = 0.566，p = 0.0003）。