Liu G, Martins I H, Chiorini J A, Davidson B L
Program in Gene Therapy, University of Iowa, Iowa City, IA 52242, USA.
Gene Ther. 2005 Oct;12(20):1503-8. doi: 10.1038/sj.gt.3302554.
The subventricular zone (SVZ) is one of the neurogenic niches in the adult mammalian brain. The SVZ is of interest for studies on neurogenesis and stem cell therapy. Here, we report specific transduction of ependyma and/or astrocytes by recombinant adeno-associated virus type 4 (AAV4) viral vectors. AAV4 vectors encoding beta-galactosidase or eGFP were injected into the lateral ventricles of neonatal and adult C57BL/6 mouse brains. In addition, SVZ injections were conducted on adult mice. AAV4 vectors show a characteristic transduction of the ependyma independent of delivery route. However, AAV4 virus injected into the SVZ targeted GFAP positive astrocytes forming the glial tube in the SVZ and rostral migratory stream (RMS). Our results introduce AAV4 as a new tool by which to manipulate glial cells in the RMS.
室下区（SVZ）是成年哺乳动物大脑中的神经源性微环境之一。室下区对于神经发生和干细胞治疗的研究具有重要意义。在此，我们报告了重组腺相关病毒4型（AAV4）病毒载体对室管膜细胞和/或星形胶质细胞的特异性转导。将编码β-半乳糖苷酶或增强型绿色荧光蛋白（eGFP）的AAV4载体注射到新生和成年C57BL/6小鼠脑的侧脑室中。此外，还对成年小鼠进行了室下区注射。AAV4载体显示出与递送途径无关的室管膜细胞特征性转导。然而，注射到室下区的AAV4病毒靶向形成室下区和吻侧迁移流（RMS）中胶质管的胶质纤维酸性蛋白（GFAP）阳性星形胶质细胞。我们的研究结果表明，AAV4是一种用于操纵吻侧迁移流中胶质细胞的新工具。
