RNA干扰对日本血吸虫体外雌虫抱雌沟蛋白基因表达的剂量依赖性抑制作用
Dose-dependent inhibition of gynecophoral canal protein gene expression in vitro in the schistosome (Schistosoma japonicum) by RNA interference.
作者信息
Cheng Guo-Feng, Lin Jiao-Jiao, Shi Yi, Jin You-Xin, Fu Zhi-Qiang, Jin Ya-Mei, Zhou Yuan-Cong, Cai You-Min
机构信息
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232, China.
出版信息
Acta Biochim Biophys Sin (Shanghai). 2005 Jun;37(6):386-90. doi: 10.1111/j.1745-7270.2005.00058.x.
Abstract
The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. This protein is widely distributed in the adult female worm after pairing. Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence were employed to analyze the relationship between the RNAi effect and dsRNA dosage in the parasites. The results revealed that the inhibition of SjGCP expression by siRNA is dose-dependent. RT-PCR analysis showed that the SjGCP transcript level was reduced by 75% when 100 nM dsRNA was applied.
摘要
日本血吸虫的雌虫抱雌沟蛋白基因SjGCP是雌雄虫配对所必需的，该基因在成年雄虫中特异性表达。配对后，这种蛋白在成年雌虫中广泛分布。采用逆转录聚合酶链反应（RT-PCR）和免疫荧光法分析寄生虫中RNA干扰效应与双链RNA剂量之间的关系。结果显示，小干扰RNA（siRNA）对SjGCP表达的抑制呈剂量依赖性。RT-PCR分析表明，当应用100 nM双链RNA时，SjGCP转录水平降低了75%。
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