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[利用噬菌体展示技术筛选日本血吸虫抱雌沟蛋白相互作用蛋白]

[Screening interaction protein of Schistosoma japonicum gynecophoral canal protein by phage display].

作者信息

He Jin-Tao, Shi Yan-Li, Liu Ping-Ping, Liu Jin-Ming, Shi Yao-Jun, Lin Jiao-Jiao, Jin Ya-Mei

机构信息

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai 200241, China.

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Jun;23(3):279-84.

Abstract

OBJECTIVE

To search the interaction protein of Schistosoma japonicum gynecophoral canal protein (SjGCP).

METHODS

The recombinant rSjGCP was used as a target to search the T7 phage display cDNA library from 44-day adult Schistosoma japonicum.

RESULTS

A total of 70 ESTs were obtained. The bioinformatics analyses to the screening results revealed that 5 interaction proteins or peptides of SjGCP were found. CONCLUSION; Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 phage display library.

摘要

目的

寻找日本血吸虫抱雌沟蛋白（SjGCP）的相互作用蛋白。

方法

以重组rSjGCP为靶标，筛选44日龄日本血吸虫成虫的T7噬菌体展示cDNA文库。

结果

共获得70个ESTs。对筛选结果的生物信息学分析显示，发现了5种SjGCP的相互作用蛋白或肽段。

结论

通过筛选T7噬菌体展示文库成功获得了SjGCP的相互作用蛋白或肽段。

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