Illés T, Dubousset J, Szendröi M, Fischer J
Department of Orthopaedics, University Medical School of Pécs, Hungary.
Pathol Res Pract. 1992 Feb;188(1-2):172-6. doi: 10.1016/S0344-0338(11)81175-1.
Lectins were used to characterize bone forming cells in posttraumatic myositis ossificans. The lectins applied were as follows: Arachis hypogaea (PNA): specific for beta-D-galactose (1,3)N-acetyl-D-galactosamine (Gal-1,3 GalNac), Canavalia ensiformis (Con A): specific for alpha-D-glucose (D-Glc) and alpha-D-mannose (D-Man) and Wheat germ (WGA): specific for N-acetyl(1,4)D-glucosamine (Glc-NaC) and neuraminic acid. The development of myositis ossificans was characterized by the appearance of a WGA binding cell population. The lectin-binding sites appeared as a cluster in the supranuclear cytoplasm, corresponding to the Golgi-complex. However, the WGA lectin-binding sites disappeared in the mature form of myositis ossificans. We assume that these lectin binding cells may be the bone marrow derived precursors of myofibroblast-like cells which are responsible for bone formation within the damaged muscle.
凝集素被用于表征创伤后骨化性肌炎中的成骨细胞。所应用的凝集素如下：花生凝集素（PNA）：对β-D-半乳糖（1,3）N-乙酰-D-半乳糖胺（Gal-1,3 GalNac）具有特异性；刀豆凝集素（Con A）：对α-D-葡萄糖（D-Glc）和α-D-甘露糖（D-Man）具有特异性；小麦胚芽凝集素（WGA）：对N-乙酰（1,4）D-葡萄糖胺（Glc-NaC）和神经氨酸具有特异性。骨化性肌炎的发展以WGA结合细胞群的出现为特征。凝集素结合位点在核上细胞质中呈簇状出现，对应于高尔基体复合体。然而，WGA凝集素结合位点在骨化性肌炎的成熟形式中消失。我们推测这些凝集素结合细胞可能是肌成纤维细胞样细胞的骨髓来源前体，它们负责受损肌肉内的骨形成。
