Agungpriyono S, Kurohmaru M, Prasetyaningtyas W E, Kaspe L, Leus K Y G, Sasaki M, Kitamura N, Yamada J, Macdonald A A
Department of Preclinical Veterinary Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Anat Histol Embryol. 2007 Oct;36(5):343-8. doi: 10.1111/j.1439-0264.2007.00778.x.
The distribution of lectin bindings in the testis of babirusa, Babyrousa babyrussa (Suidae) was studied histochemically using 10 biotinylated lectins, Peanut agglutinin (PNA), Ricinus communis agglutinin (RCA I), Dolichos biflorus agglutinin (DBA), Vicia villosa agglutinin (VVA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Lens culinaris agglutinin (LCA), Pisum sativum agglutinin (PSA), Concanavalin A(Con A) and Ulex europaeus agglutinin (UEA I). Nine of 10 lectins showed a variety of staining patterns in the seminiferous epithelium and interstitial cells. The acrosome of Golgi-, cap- and acrosome-phase spermatids displayed various PNA, RCA I, VVA, SBA and WGA bindings, indicating the presence of glycoconjugates with D-galactose, N-acetyl-D-galactosamine and N-acetyl-D-glucosamine sugar residues respectively. No affinity was detected in the acrosome of late spermatids. LCA, PSA and Con A which have affinity for D-mannose and D-glucose sugar residues were positive in the cytoplasm of spermatids and spermatocytes. DBA was positive only in spermatogonia. In addition to DBA, positive binding in spermatogonia was found for VVA, WGA and Con A, suggesting the distribution of glycoconjugates with N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-mannose and D-glucose sugar residues. Sertoli cells were stained intensely with RCA I, WGA and Con A. In Leydig cells, RCA I and Con A were strongly positive, while WGA, LCA and PSA reactions were weak to moderate. The present findings showed that the distribution pattern of lectin binding in the testis of babirusa is somewhat different from that of pig or other mammals reported previously.
利用10种生物素化凝集素，即花生凝集素（PNA）、蓖麻凝集素（RCA I）、双花扁豆凝集素（DBA）、绒毛豌豆凝集素（VVA）、大豆凝集素（SBA）、麦胚凝集素（WGA）、菜豆凝集素（LCA）、豌豆凝集素（PSA）、伴刀豆球蛋白A（Con A）和荆豆凝集素（UEA I），采用组织化学方法研究了鹿豚（猪科）睾丸中凝集素结合的分布情况。10种凝集素中有9种在生精上皮和间质细胞中呈现出多种染色模式。高尔基期、帽期和顶体期精子细胞的顶体显示出不同的PNA、RCA I、VVA、SBA和WGA结合，分别表明存在含有D - 半乳糖、N - 乙酰 - D - 半乳糖胺和N - 乙酰 - D - 葡萄糖胺糖残基的糖缀合物。在晚期精子细胞的顶体中未检测到亲和力。对D - 甘露糖和D - 葡萄糖糖残基有亲和力的LCA、PSA和Con A在精子细胞和精母细胞的细胞质中呈阳性。DBA仅在精原细胞中呈阳性。除DBA外，在精原细胞中还发现VVA、WGA和Con A呈阳性结合，表明存在含有N - 乙酰 - D - 半乳糖胺、N - 乙酰 - D - 葡萄糖胺、D - 甘露糖和D - 葡萄糖糖残基的糖缀合物。支持细胞被RCA I、WGA和Con A强烈染色。在睾丸间质细胞中，RCA I和Con A呈强阳性，而WGA、LCA和PSA反应为弱阳性至中度阳性。目前的研究结果表明，鹿豚睾丸中凝集素结合的分布模式与先前报道的猪或其他哺乳动物的有所不同。
