Díaz-Güemes Martín-Portugués I, Sánchez Margallo F M, Pascual Sánchez-Gijón S, Crisóstomo Ayala V, Usón Gargallo J
Minimally Invasive Surgery Centre, Cáceres, Spain.
Histol Histopathol. 2005 Jul;20(3):851-6. doi: 10.14670/HH-20.851.
This paper describes the histological features of the vagus nerve after its stimulation with an electrostimulation system that is being developed for morbid obesity treatment. An electrostimulation system was implanted laparoscopically around the ventral vagal trunk of five Large White female pigs (49.63+/-1.94 kg.). Vagal nerve stimulation was performed by continuous constant voltage current pulses. Thoracic samples of both ventral and dorsal vagal trunks were obtained thoracoscopically one month after implantation. Animals were sacrificed one month after thoracoscopic vaguectomy. Tissue samples were then harvested from the vagal nerve at the implantation site, 1cm cranial to it, thoracic portion of ventral and dorsal vagal trunks, sub-diaphragmatic dorsal vagal trunk, left and right vagus nerves. Specimens were analysed with light microscope. The severity of the lesions was graded from 0 to 4 (0: no lesion, 1: mild, 2: moderate, 3: severe and 4: extremely severe), taking into account fibrosis, vascularization, necrosis, fiber degeneration and inflammation. Electrode implantation resulted in thickened epineurium and endoneural connective tissue. The greatest lesion score was evidenced at the leads implantation site in the ventral vagal trunk, followed by, in order of decreasing lesion severity, left vagus nerve, thoracic portion of ventral vagal trunk, subdiaphragmatic dorsal vagal trunk, thoracic portion of dorsal vagal trunk and right vagus nerve. The stimulation device used in this study caused connective tissue growth, greatest in the samples located closer to the implantation site. However, there was no sign of altered vascularization in any studied specimen.
本文描述了使用一种正在研发用于治疗病态肥胖的电刺激系统刺激迷走神经后的组织学特征。将一个电刺激系统经腹腔镜植入5头大白母猪（体重49.63±1.94千克）腹侧迷走神经干周围。通过连续恒压电流脉冲进行迷走神经刺激。植入后1个月，通过胸腔镜获取腹侧和背侧迷走神经干的胸部样本。在胸腔镜下进行迷走神经切除术后1个月处死动物。然后从植入部位的迷走神经、其头侧1厘米处、腹侧和背侧迷走神经干的胸部部分、膈下背侧迷走神经干、左右迷走神经采集组织样本。用光学显微镜分析标本。根据纤维化、血管化、坏死、纤维变性和炎症情况，将病变严重程度分为0至4级（0：无病变，1：轻度，2：中度，3：重度，4：极重度）。电极植入导致神经外膜和神经内膜结缔组织增厚。腹侧迷走神经干的电极植入部位病变评分最高，其次按病变严重程度递减顺序依次为左迷走神经、腹侧迷走神经干胸部部分、膈下背侧迷走神经干、背侧迷走神经干胸部部分和右迷走神经。本研究中使用的刺激装置导致结缔组织生长，在离植入部位较近的样本中最为明显。然而，在任何研究标本中均未发现血管化改变的迹象。
