Turhani Dritan, Cvikl Barbara, Watzinger Elisabeth, Weissenböck Martina, Yerit Kaan, Thurnher Dietmar, Lauer Günter, Ewers Rolf
Medical University of Vienna, Hospital of Cranio-Maxillofacial and Oral Surgery, Austria.
J Oral Maxillofac Surg. 2005 Jun;63(6):793-9. doi: 10.1016/j.joms.2005.02.015.
The purpose of this study was to analyze the interaction between osteoblast-like cells isolated from mandibular bone and hydroxyapatite ceramic bone substitute obtained from calcified red algae to assess the growth and differentiation of adherent cells on this biomaterial.
The macroporous ceramic material C GRAFT/Algipore (The Clinician's Preference LLC, Golden, CO) is composed of 100% hydroxyapatite and possesses specific mechanical and physiochemical properties. Osteoblast-like cells were seeded on 200 mg of biomaterial and cultured for 6 and 21 days under osteogenic differentiation conditions. Specific alkaline phosphatase activity, DNA, and protein content of the proliferating cells were analyzed. The morphology of the cells in contact with the biomaterial was examined by scanning electron microscopy. The osteoblastic phenotype of the cells was confirmed by analysis of the expression of bone-specific genes (osteocalcin, osteopontin and collagen type I) by semi-quantitative reverse transcriptase polymerase chain reaction.
The DNA and protein content increased over the culture period. Scanning electron microscopy showed cells spreading on the surface of the biomaterials, covering the macropores, and colonizing the depth of the particles. The analysis of the expression patterns of bone-related genes confirmed the osteoblastic phenotype of the cultured cells.
The results of this study showed that hydroxyapatite ceramic bone substitute obtained from calcified red algae support the proliferation and differentiation of human osteoblast-like cells on its surface in vitro and might be suitable for use as scaffolds in tissue engineering strategies in vivo.
本研究旨在分析从下颌骨分离的成骨样细胞与从钙化红藻获得的羟基磷灰石陶瓷骨替代物之间的相互作用，以评估贴壁细胞在这种生物材料上的生长和分化情况。
大孔陶瓷材料C GRAFT/Algipore（The Clinician's Preference LLC，科罗拉多州戈尔登）由100%羟基磷灰石组成，具有特定的力学和物理化学性质。将成骨样细胞接种在200mg生物材料上，并在成骨分化条件下培养6天和21天。分析增殖细胞的特异性碱性磷酸酶活性、DNA和蛋白质含量。通过扫描电子显微镜检查与生物材料接触的细胞形态。通过半定量逆转录聚合酶链反应分析骨特异性基因（骨钙素、骨桥蛋白和I型胶原）的表达，以确认细胞的成骨细胞表型。
在培养期间，DNA和蛋白质含量增加。扫描电子显微镜显示细胞在生物材料表面铺展，覆盖大孔，并在颗粒深处定植。对骨相关基因表达模式的分析证实了培养细胞的成骨细胞表型。
本研究结果表明，从钙化红藻获得的羟基磷灰石陶瓷骨替代物在体外支持人成骨样细胞在其表面的增殖和分化，可能适合用作体内组织工程策略中的支架。
