Baldini G, Hohl T, Lin H Y, Lodish H F
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142.
Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5049-52. doi: 10.1073/pnas.89.11.5049.
We have isolated the cDNA for Rab3D, an additional member of the small molecular weight GTP-binding protein family. Rab3D message is abundant in mouse adipocytes. It is increased during differentiation of 3T3-L1 cells into adipocytes, temporally coincident with the appearance of the insulin-sensitive glucose transporter GLUT4. Rab3D is a close homolog of Rab3A, which is found on the cytoplasmic surface of neurosecretory vesicles and which may be involved in their regulated secretion. Since our previous work showed that in permeabilized adipocytes nonhydrolizable GTP analogs mimic insulin in triggering exocytosis of GLUT4-containing vesicles, Rab3D may be involved in the insulin-induced exocytosis of GLUT4-containing vesicles in adipocytes.
我们已经分离出Rab3D的互补DNA（cDNA），它是小分子量GTP结合蛋白家族的另一个成员。Rab3D信息在小鼠脂肪细胞中含量丰富。在3T3-L1细胞分化为脂肪细胞的过程中它会增加，在时间上与胰岛素敏感的葡萄糖转运蛋白GLUT4的出现一致。Rab3D是Rab3A的紧密同源物，Rab3A存在于神经分泌小泡的细胞质表面，可能参与其调节性分泌。由于我们之前的研究表明，在通透的脂肪细胞中，不可水解的GTP类似物在触发含GLUT4小泡的胞吐作用方面模拟胰岛素，Rab3D可能参与脂肪细胞中胰岛素诱导的含GLUT4小泡的胞吐作用。
