Birnbaum M J, Haspel H C, Rosen O M
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5784-8. doi: 10.1073/pnas.83.16.5784.
Antibody raised against the human erythrocyte glucose transporter identified a recombinant lambda gt11 bacteriophage in a cDNA library prepared from immunoselected polysomal RNA from adult rat brain. The cDNA predicts a 492-amino acid protein that demonstrates 97.6% identity to the human hepatoma hexose carrier. The tissue distribution of the transporter mRNA is identical to that of immunologically identifiable protein and transport activity, except in liver in which high levels of transport are associated with little or no transporter mRNA or protein. As assayed by blot-hybridization analysis, mRNA from insulin-responsive and nonresponsive tissues are indistinguishable. These data suggest that a genetically unrelated protein is responsible for hexose transport in normal liver.
针对人类红细胞葡萄糖转运蛋白产生的抗体，在从成年大鼠脑免疫筛选的多核糖体RNA构建的cDNA文库中鉴定出一种重组λgt11噬菌体。该cDNA预测一种492个氨基酸的蛋白质，它与人类肝癌己糖载体的同源性为97.6%。除了肝脏中高水平的转运与很少或没有转运蛋白mRNA或蛋白质相关外，转运蛋白mRNA的组织分布与免疫可识别的蛋白质和转运活性的分布相同。通过印迹杂交分析检测，来自胰岛素反应性和非反应性组织的mRNA没有区别。这些数据表明，一种遗传上不相关的蛋白质负责正常肝脏中的己糖转运。
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