二甲双胍和苯乙双胍对甲基乙二醛形成晚期糖基化终末产物的影响。
Effect of buformin and metformin on formation of advanced glycation end products by methylglyoxal.
作者信息
Kiho Tadashi, Kato Motohiro, Usui Shigeyuki, Hirano Kazuyuki
机构信息
Gifu Prefectural Institute of Health and Environmental Sciences, 1-1 Naka-fudogaoka, Kakamigahara 504-0838, Japan.
出版信息
Clin Chim Acta. 2005 Aug;358(1-2):139-45. doi: 10.1016/j.cccn.2005.02.012.
BACKGROUND
The formation and accumulation of advanced glycation end products (AGE) in various tissues are known to be involved in the aging process and complications of long-term diabetes. Aminoguanidine as AGE inhibitors was first studied, and metformin as biguanide compounds have been reported to react with reactive dicarbonyl precursors such as methylglyoxal.
METHODS
We studied the effects of the biguanides of buformin and metformin on AGE formation by the methods of specific fluorescence, and enzyme-linked immunosorbent assay and a Western blot analysis using the anti-AGE antibody after incubating BSA or RNase with methylglyoxal.
RESULTS
Buformin is a more potent inhibitor of AGE formation than metformin, and suggests that the amino group of buformin trap the carbonyl group of methylglyoxal to suppress formation of AGE.
CONCLUSION
In addition to that of metformin, buformin may be clinically useful to prevent diabetic complications.
背景
已知各种组织中晚期糖基化终产物（AGE）的形成和积累与衰老过程及长期糖尿病并发症有关。氨基胍作为AGE抑制剂最早被研究，而二甲双胍作为双胍类化合物已被报道可与活性二羰基前体如甲基乙二醛发生反应。
方法
我们通过特异性荧光法、酶联免疫吸附测定法以及在牛血清白蛋白（BSA）或核糖核酸酶（RNase）与甲基乙二醛孵育后使用抗AGE抗体进行蛋白质印迹分析，研究了丁福明和二甲双胍这两种双胍类药物对AGE形成的影响。
结果
丁福明比二甲双胍更有效地抑制AGE的形成，这表明丁福明的氨基基团捕获了甲基乙二醛的羰基基团以抑制AGE的形成。
结论
除二甲双胍外，丁福明在临床上可能对预防糖尿病并发症有用。
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