Department of Pharmacognosy and Herbal Medicines, Faculty of Pharmacy, Wroclaw Medical University, ul. Borowska 211, 50-556 Wrocław, Poland.
Laboratory of Chemometrics and Applied Spectroscopy, Department of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383 Wrocław, Poland.
Molecules. 2022 Apr 9;27(8):2432. doi: 10.3390/molecules27082432.
Reactive oxygen and carbonyl species promote oxidative and carbonyl stress, and the development of diabetes, metabolic syndrome, cardiovascular diseases, and others. The traditional herb is known for its antioxidant properties; therefore, the current study aimed to assess how the chemical composition of a water infusion corresponds with its antioxidative and antiglycative effects in vitro. The composition of infusions prepared from commercial products was analyzed with UHPLC-ESI-qTOF-MS. Total phenolics, flavonoids, and non-flavonoid polyphenols were determined. Antioxidant activity of infusions and selected polyphenols was investigated using DPPH, ABTS, and FRAP. Fluorometric measurements and methylglyoxal capture were performed to investigate the antiglycation activity. PCA and PLS-DA models were applied to explore the correlation between chemical and antioxidant results. The principal flavonoids in were flavonols. In vitro tests revealed that a stronger antioxidant effect was demonstrated by plant material from Turkey rich in flavonoids, followed by Albania and Greece. Flavonols and ellagic acid displayed stronger antiradical and reducing power than EA-derived urolithins. Hyperoside was the most potent inhibitor of glycation. The results indicate that flavonoids are primarily responsible for rock rose antioxidant and antiglycation properties. PLS-DA modeling can be used to identify the origin of plant material with sensitivity and specificity exceeding 86%.
活性氧和羰基物种会促进氧化和羰基应激，从而导致糖尿病、代谢综合征、心血管疾病等疾病的发生。传统草药以其抗氧化特性而闻名；因此，本研究旨在评估水浸剂的化学成分与其体外抗氧化和抗糖化作用之间的关系。采用 UHPLC-ESI-qTOF-MS 分析了来自商业产品的浸剂的成分。测定了浸剂和选定多酚的总酚、类黄酮和非类黄酮多酚含量。采用 DPPH、ABTS 和 FRAP 法研究了浸剂和选定多酚的抗氧化活性。通过荧光测量和甲基乙二醛捕获来研究抗糖化活性。应用 PCA 和 PLS-DA 模型来探索化学和抗氧化结果之间的相关性。是主要的类黄酮为黄酮醇。体外试验表明，富含类黄酮的土耳其植物材料表现出更强的抗氧化作用，其次是阿尔巴尼亚和希腊。黄酮醇和鞣花酸的自由基清除能力和还原能力强于 EA 衍生的 urolithins。桃叶珊瑚苷是最强的糖化抑制剂。结果表明，类黄酮主要负责香桃木的抗氧化和抗糖化特性。PLS-DA 模型可用于鉴定植物材料的来源，其灵敏度和特异性超过 86%。
