Zhao J, Li S P, Yang F Q, Li P, Wang Y T
Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau.
J Chromatogr A. 2006 Mar 10;1108(2):188-94. doi: 10.1016/j.chroma.2005.12.104. Epub 2006 Feb 3.
The seed of Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou, Suanzaoren in Chinese, is one of commonly used Chinese medicines. Saponins and fatty oil contains several fatty acids in Suanzaoren are responsible for its therapeutic activities. In this study, a new HPLC coupled with evaporative light scattering detection (ELSD) and pressurized liquid extraction (PLE) method was developed for the simultaneous quantitative determination of 11 major components of 2 saponins and 9 fatty acids, namely jujuboside A, jujuboside B, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, arachidic acid and docosanoic acid in Suanzaoren. Simultaneous separation of these eleven compounds was achieved on a C18 analytical column. The mobile phase consisted of (A) 0.1% aqueous acetic acid and (B) methanol with 0.1% acetic acid using a gradient elution. The drift tube temperature of ELSD was set at 75 degrees C, and nitrogen flow-rate was 1.8l/min. All calibration curves showed good linearity (r(2)>0.9955) within test ranges. This method showed good reproducibility for the quantification of these eleven components in Suanzaoren with intra- and inter-day variations of less than 3.41 and 4.37%, respectively. The validated method was successfully applied to quantify 11 investigated components in nine commercial samples of Suanzaoren.
酸枣仁为鼠李科植物酸枣Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou的干燥成熟种子，是常用的中药材之一。酸枣仁中的皂苷类和含有多种脂肪酸的脂肪油是其发挥治疗作用的物质基础。本研究建立了高效液相色谱-蒸发光散射检测法（HPLC-ELSD）结合加压液体萃取法（PLE），用于同时定量测定酸枣仁中2种皂苷和9种脂肪酸共11种主要成分，即酸枣仁皂苷A、酸枣仁皂苷B、月桂酸、肉豆蔻酸、棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸、花生酸和二十二烷酸。在C18分析柱上实现了这11种化合物的同时分离。流动相由（A）0.1%乙酸水溶液和（B）含0.1%乙酸的甲醇组成，采用梯度洗脱。蒸发光散射检测器的漂移管温度设定为75℃，氮气流量为1.8l/min。所有校准曲线在测试范围内均呈现良好的线性关系（r(2)>0.9955）。该方法用于酸枣仁中这11种成分的定量分析时具有良好的重现性，日内和日间变化分别小于3.41%和4.37%。该验证方法成功应用于9个市售酸枣仁商品样品中11种被测成分的定量分析。
