Palchaudhuri Monika, Flügge Gabriele
Clinical Neurobiology Laboratory, German Primate Center, Göttingen.
Cell Tissue Res. 2005 Aug;321(2):159-72. doi: 10.1007/s00441-005-1112-x. Epub 2005 Jun 10.
We studied expression of the 5-HT(1A) receptor in cortical and limbic areas of the brain of the tree shrew. In situ hybridization with a receptor-specific probe and immunocytochemistry with various antibodies was used to identify distinct neurons expressing the receptor. In vitro receptor autoradiography with (3)H-8-OH-DPAT ((3)H-8-hydroxy-2-[di-n-propylamino]tetralin) was performed to visualize receptor-binding sites. In the prefrontal, insular, and occipital cortex, 5-HT(1A) receptor mRNA was expressed in pyramidal neurons of layer 2, whereas (3)H-8-OH-DPAT labeled layers 1 and 2 generating a columnar-like pattern in the prefrontal and occipital cortex. In the striate and ventral occipital cortex, receptor mRNA was present within layers 5 and 6 in pyramidal neurons and Meynert cells. Pyramid-like neurons in the claustrum and anterior olfactory nucleus also expressed the receptor. Principal neurons in hippocampal region CA1 expressed 5-HT(1A) receptor mRNA, and (3)H-8-OH-DPAT labeled both the stratum oriens and stratum radiatum. CA3 pyramidal neurons displayed low 5-HT(1A) receptor expression, whereas granule neurons in the dentate gyrus revealed moderate expression of this receptor. In the amygdala, large pyramid-like neurons in the basal magnocellular nucleus strongly expressed the receptor. Immunocytochemistry with antibodies against parvalbumin, calbindin, and gamma aminobutyric acid (GABA) provided no evidence for 5-HT(1A) receptor expression in GABAergic neurons in cortical and limbic brain areas. Our data agree with previous findings showing that the 5-HT(1A) receptor mediates the modulation of glutamatergic neurons. Expression in the limbic and cortical areas suggested an involvement of 5-HT(1A) receptors in emotional and cognitive processes.
我们研究了树鼩大脑皮质和边缘区域中5-羟色胺（5-HT）1A受体的表达情况。使用受体特异性探针进行原位杂交，并使用各种抗体进行免疫细胞化学，以鉴定表达该受体的不同神经元。采用（3）H-8-OH-DPAT（（3）H-8-羟基-2-[二正丙基氨基]四氢萘）进行体外受体放射自显影，以可视化受体结合位点。在额叶、岛叶和枕叶皮质，5-HT1A受体mRNA在第2层的锥体神经元中表达，而（3）H-8-OH-DPAT标记第1层和第2层，在额叶和枕叶皮质形成柱状样模式。在纹状皮质和枕叶腹侧皮质，受体mRNA存在于第5层和第6层的锥体神经元和迈内特细胞中。屏状核和前嗅核中的金字塔样神经元也表达该受体。海马区域CA1的主要神经元表达5-HT1A受体mRNA，（3）H-8-OH-DPAT标记了海马下脚和辐射层。CA3锥体神经元显示出低水平的5-HT1A受体表达，而齿状回中的颗粒神经元则显示出该受体的中度表达。在杏仁核中，基底大细胞核中的大型金字塔样神经元强烈表达该受体。使用抗小白蛋白、钙结合蛋白和γ-氨基丁酸（GABA）抗体进行的免疫细胞化学未提供皮质和边缘脑区GABA能神经元中5-HT1A受体表达的证据。我们的数据与先前的研究结果一致，表明5-HT1A受体介导谷氨酸能神经元的调节。在边缘和皮质区域的表达表明5-HT1A受体参与情绪和认知过程。
