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来自巴西的犬肝簇虫物种的分子鉴定与特征分析。

Molecular identification and characterization of canine Hepatozoon species from Brazil.

作者信息

Rubini Adriano Stefani, dos Santos Paduan Karina, Cavalcante Gustavo Góes, Ribolla Paulo Eduardo Martins, O'Dwyer Lucia Helena

机构信息

Instituto de Biociências, Departamento de Parasitologia, Universidade Estadual Paulista (UNESP), 18618-000, Botucatu, São Paulo, Brazil.

出版信息

Parasitol Res. 2005 Sep;97(2):91-3. doi: 10.1007/s00436-005-1383-x. Epub 2005 Jun 10.

Abstract

Canine Hepatozoon species from Brazil was molecular identified and characterized for the first time. From 31 dogs, 7 were positive for blood smear examination and 21 positive for PCR. Partial sequences of the 18S rRNA gene from eight naturally infected dogs were analyzed. Sequences revealed that Brazilian Hepatozoon is closely related with the Japanese Hepatozoon, that has 99% nucleotide identity with Hepatozoon canis from Israel, and different from Hepatozoon americanum. These results indicate that the canine Hepatozoon species from Brazil is H. canis.

摘要

来自巴西的犬肝簇虫首次通过分子方法进行了鉴定和特征分析。在31只犬中,7只血液涂片检查呈阳性,21只PCR检测呈阳性。对8只自然感染犬的18S rRNA基因部分序列进行了分析。序列显示,巴西肝簇虫与日本肝簇虫密切相关,与来自以色列的犬肝簇虫有99%的核苷酸同一性,且与美洲肝簇虫不同。这些结果表明,来自巴西的犬肝簇虫种类为犬肝簇虫。

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