α-连环蛋白的表达在胃癌患者中降低。

Alpha-catenin expression is decreased in patients with gastric carcinoma.

作者信息

Zhou Yong-Ning, Xu Cai-Pu, Chen Yu, Han Biao, Yang Shi-Ming, Fang Dian-Chun

机构信息

Department of Gastroenterology, First Hospital, Lanzhou University, Lanzhou 730000, Gansu Province, China.

出版信息

World J Gastroenterol. 2005 Jun 14;11(22):3468-72. doi: 10.3748/wjg.v11.i22.3468.

DOI:10.3748/wjg.v11.i22.3468

PMID:15948257

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4316006/

Abstract

AIM

To assess the expression of alpha-catenin in gastric carcinoma and to determine the role of alpha-catenin expression in gastric carcinogenesis.

METHODS

alpha-catenin expression was assessed by semi-quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical staining in 49 gastric carcinomas, 26 adjacent non-cancerous mucosae, and gastric biopsy specimens from 11 healthy controls.

RESULTS

mRNA levels of alpha-catenin were reduced or absent in 34 of 49 (69%) gastric carcinoma tissues and in 5 of 26 (19%) tumor-free gastric mucosae of carcinoma patients, respectively. Of the carcinoma samples with altered alpha-catenin mRNA levels, alpha-catenin expression was negative in 20 and decreased in 14 cases. Up to 69% of tumors were stained abnormally for alpha-catenin. Of the 34 cases whose mRNA expression of alpha-catenin was reduced, 32 (94%) showed abnormal immunostaining patterns, while only 2 showed a normal alpha-catenin expression. The frequency of reduced expression of alpha-catenin mRNA was 14% in well-differentiated carcinomas, higher than that in poorly differentiated carcinomas (86%). A significant correlation was not shown between alpha-catenin expression and both depth of invasion and lymph node metastasis. Moreover, there was no statistical difference between loss or down-regulation of alpha-catenin mRNA and Helicobacter pylori (H. pylori) infection.

CONCLUSION

Downregulation of alpha-catenin expression is common in gastric carcinoma, and alpha-catenin expression may be used as a differentiation marker. Downregulation of alpha-catenin expression may be an early event in tumorigenesis. Reduced alpha-catenin expression is not correlated with H. pylori infection.

摘要

目的

评估α-连环蛋白在胃癌中的表达，并确定α-连环蛋白表达在胃癌发生中的作用。

方法

采用半定量逆转录聚合酶链反应和免疫组织化学染色法，对49例胃癌、26例癌旁非癌黏膜以及11例健康对照者的胃活检标本进行α-连环蛋白表达评估。

结果

49例胃癌组织中有34例（69%）α-连环蛋白mRNA水平降低或缺失，癌旁无肿瘤的胃癌患者胃黏膜中有5例（19%）α-连环蛋白mRNA水平降低或缺失。在α-连环蛋白mRNA水平改变的癌组织样本中，20例α-连环蛋白表达阴性，14例表达降低。高达69%的肿瘤α-连环蛋白染色异常。在α-连环蛋白mRNA表达降低的34例病例中，32例（94%）免疫染色模式异常，仅2例α-连环蛋白表达正常。高分化癌中α-连环蛋白mRNA表达降低的频率为14%，高于低分化癌（86%）。α-连环蛋白表达与浸润深度和淋巴结转移均无显著相关性。此外，α-连环蛋白mRNA缺失或下调与幽门螺杆菌（H. pylori）感染之间无统计学差异。