α-连环蛋白在人类胃癌及一级亲属的胃黏膜中表达降低。

alpha-catenin expression is decreased in human gastric cancers and in the gastric mucosa of first degree relatives.

作者信息

Yu J, Ebert M P, Miehlke S, Rost H, Lendeckel U, Leodolter A, Stolte M, Bayerdörffer E, Malfertheiner P

机构信息

Department of Gastroenterology, Hepatology, and Infectious Diseases, Otto-von-Guericke University, D-39120 Magdeburg, Germany.

出版信息

Gut. 2000 May;46(5):639-44. doi: 10.1136/gut.46.5.639.

DOI:10.1136/gut.46.5.639

PMID:10764706

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1727909/

Abstract

BACKGROUND AND AIMS

The role of altered cell adhesion is critical for the development of epithelial cancers. E-cadherin plays an important role in the maintenance of cell-cell adhesion and its function is thought to be regulated by its associated cytoplasmic proteins, such as alpha-catenin and beta-catenin. To determine the role of alpha-catenin expression in gastric carcinogenesis, we studied its expression in human gastric cancer and in the gastric mucosa of first degree relatives with no clinical disease.

METHODS

alpha-Catenin expression was assessed by immunohistochemical analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) using gastric tissue specimens from patients with gastric cancer and from the gastric mucosa of first degree relatives of gastric cancer patients and healthy controls.

RESULTS

mRNA levels of alpha-catenin were reduced or absent in 13 of 19 gastric cancer tissues, which differed significantly from levels found in the tumour free gastric mucosa of cancer patients (p<0.05). Of the cancer samples with altered alpha-catenin mRNA levels, alpha-catenin expression was negative in seven and decreased in six cases. Interestingly, decreased alpha-catenin mRNA expression also occurred in the mucosa of the corpus (11/18) and antrum (4/18) of first degree relatives. In the corpus biopsies alpha-catenin expression was more often decreased or lost compared with the antrum biopsies in first degree relatives and healthy controls (p<0.05). Immunohistochemical analysis revealed membranous expression of alpha-catenin in gastric cancer cells and the non-malignant gastric epithelium. However, some cancers also exhibited loss of membranous staining. Generally, loss or downregulation of alpha-catenin mRNA in the gastric mucosa was associated with Helicobacter pylori infection (p<0.05).

CONCLUSION

Our findings suggest that loss or downregulation of alpha-catenin expression may be an early event in gastric carcinogenesis and may be associated with H pylori infection.

摘要

背景与目的

细胞黏附改变在上皮癌发生过程中起关键作用。E-钙黏蛋白在维持细胞间黏附中起重要作用，其功能被认为受相关胞质蛋白如α-连环蛋白和β-连环蛋白调控。为确定α-连环蛋白表达在胃癌发生中的作用，我们研究了其在人类胃癌及无临床疾病的一级亲属胃黏膜中的表达情况。

方法

采用免疫组织化学分析及逆转录聚合酶链反应（RT-PCR），利用胃癌患者的胃组织标本、胃癌患者一级亲属及健康对照者的胃黏膜标本评估α-连环蛋白表达。

结果

19例胃癌组织中有13例α-连环蛋白的mRNA水平降低或缺失，这与癌患者无肿瘤的胃黏膜中发现的水平有显著差异（p<0.05）。在α-连环蛋白mRNA水平改变的癌样本中，α-连环蛋白表达7例为阴性，6例降低。有趣的是，一级亲属胃体（11/18）和胃窦（4/18）的黏膜中也出现α-连环蛋白mRNA表达降低。在一级亲属和健康对照者中，胃体活检标本中α-连环蛋白表达比胃窦活检标本更常降低或缺失（p<0.05）。免疫组织化学分析显示α-连环蛋白在胃癌细胞及非恶性胃上皮中呈膜性表达。然而，一些癌症也表现出膜性染色缺失。一般来说，胃黏膜中α-连环蛋白mRNA的缺失或下调与幽门螺杆菌感染相关（p<0.05）。

结论

我们的研究结果表明，α-连环蛋白表达的缺失或下调可能是胃癌发生的早期事件，且可能与幽门螺杆菌感染有关。

相似文献

alpha-catenin expression is decreased in human gastric cancers and in the gastric mucosa of first degree relatives.

Gut. 2000 May;46(5):639-44. doi: 10.1136/gut.46.5.639.

Gastric mucosa alterations in first-degree relatives of gastric cancer patients.

Anticancer Res. 2005 May-Jun;25(3c):2567-72.

Effect of eradication of Helicobacter pylori on expression levels of FHIT, IL-8 and P73 in gastric mucosa of first-degree relatives of gastric cancer patients.

PLoS One. 2015 Apr 14;10(4):e0124576. doi: 10.1371/journal.pone.0124576. eCollection 2015.

Alpha-catenin expression is decreased in patients with gastric carcinoma.

World J Gastroenterol. 2005 Jun 14;11(22):3468-72. doi: 10.3748/wjg.v11.i22.3468.

Ghrelin in diseases of the gastric mucosa associated with Helicobacter pylori infection.

Med Sci Monit. 2010 Oct;16(10):CR493-500.

Helicobacter pylori infection and family history of gastric cancer decrease expression of FHIT tumor suppressor gene in gastric mucosa of dyspeptic patients.

Helicobacter. 2009 Oct;14(5):126-34. doi: 10.1111/j.1523-5378.2009.00715.x.

Helicobacter pylori-induced epithelial-mesenchymal transition, a potential role of gastric cancer initiation and an emergence of stem cells.

Carcinogenesis. 2015 May;36(5):553-63. doi: 10.1093/carcin/bgv022. Epub 2015 Mar 16.

Difference of p53AIP1 mRNA expression in gastric mucosa between patients with gastric cancer and chronic gastritis infected with Helicobacter pylori.

J Clin Gastroenterol. 2008 Apr;42(4):351-5. doi: 10.1097/MCG.0b013e318054493e.

Increased beta-catenin mRNA levels and mutational alterations of the APC and beta-catenin gene are present in intestinal-type gastric cancer.

Carcinogenesis. 2002 Jan;23(1):87-91. doi: 10.1093/carcin/23.1.87.

Downregulated Expression of E-cadherin and TP53 in Patients with Gastric Diseases: the Involvement of H. pylori Infection and Its Virulence Markers.

J Gastrointest Cancer. 2016 Mar;47(1):20-6. doi: 10.1007/s12029-015-9782-4.

引用本文的文献

The Role of CTNNA1 in Malignancies: An Updated Review.

J Cancer. 2023 Jan 1;14(2):219-230. doi: 10.7150/jca.79236. eCollection 2023.

The Chemical Potential of Plasma Membrane Cholesterol: Implications for Cell Biology.

Biophys J. 2018 Feb 27;114(4):904-918. doi: 10.1016/j.bpj.2017.12.042.

Bifidobacterium bifidum PRL2010 modulates the host innate immune response.

Appl Environ Microbiol. 2014 Jan;80(2):730-40. doi: 10.1128/AEM.03313-13. Epub 2013 Nov 15.

Loss of alpha-catenin decreases the strength of single E-cadherin bonds between human cancer cells.

J Biol Chem. 2009 Jul 3;284(27):18252-9. doi: 10.1074/jbc.M109.000661. Epub 2009 May 19.

Bench to bedside and back again: molecular mechanisms of alpha-catenin function and roles in tumorigenesis.

Semin Cancer Biol. 2008 Feb;18(1):53-64. doi: 10.1016/j.semcancer.2007.08.003. Epub 2007 Sep 4.

Syndrome differentiation in traditional Chinese medicine and E-cadherin/ICAM-1 gene protein expression in gastric carcinoma.

World J Gastroenterol. 2007 Aug 28;13(32):4321-7. doi: 10.3748/wjg.v13.i32.4321.

Elevated risk for gastric adenocarcinoma can be predicted from histomorphology.

World J Gastroenterol. 2006 Oct 14;12(38):6109-14. doi: 10.3748/wjg.v12.i38.6109.

Eradication of H pylori for the prevention of gastric cancer.

World J Gastroenterol. 2006 Aug 28;12(32):5101-7. doi: 10.3748/wjg.v12.i32.5101.

Effects of Helicobacter pylori on the cadherin-catenin complex.

J Clin Pathol. 2006 Dec;59(12):1261-6. doi: 10.1136/jcp.2006.036772. Epub 2006 May 5.

Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas.

World J Gastroenterol. 2006 Apr 14;12(14):2168-73. doi: 10.3748/wjg.v12.i14.2168.

本文引用的文献

Studies of the hormonal regulation of type 2 5'-iodothyronine deiodinase messenger ribonucleic acid in pituitary tumor cells using semiquantitative reverse transcription-polymerase chain reaction.

Endocrinology. 1998 Dec;139(12):4895-905. doi: 10.1210/endo.139.12.6334.

Mutations of the human E-cadherin (CDH1) gene.

Hum Mutat. 1998;12(4):226-37. doi: 10.1002/(SICI)1098-1004(1998)12:4<226::AID-HUMU2>3.0.CO;2-D.

Loss of beta1 integrin function results in a retardation of myogenic, but an acceleration of neuronal, differentiation of embryonic stem cells in vitro.

Dev Biol. 1998 Sep 15;201(2):167-84. doi: 10.1006/dbio.1998.9002.

Increased cell proliferation of the gastric mucosa in first-degree relatives of gastric carcinoma patients.

Cancer. 1998 Sep 1;83(5):876-81.

H pylori infection is associated with downregulation of E-cadherin, a molecule involved in epithelial cell adhesion and proliferation control.

J Clin Pathol. 1998 May;51(5):410-2. doi: 10.1136/jcp.51.5.410.

The cadherin superfamily at the synapse: more members, more missions.

Cell. 1998 Jun 26;93(7):1095-8. doi: 10.1016/s0092-8674(00)81452-x.

E-cadherin germline mutations in familial gastric cancer.

Nature. 1998 Mar 26;392(6674):402-5. doi: 10.1038/32918.

The importance of the E-cadherin-catenin complex in the maintenance of intestinal epithelial homoeostasis: more than intercellular glue?

Gut. 1997 Nov;41(5):581-4. doi: 10.1136/gut.41.5.581.

Histological diagnosis of Helicobacter pylori gastritis is predictive of a high risk of gastric carcinoma.

Int J Cancer. 1997 Dec 10;73(6):837-9. doi: 10.1002/(sici)1097-0215(19971210)73:6<837::aid-ijc12>3.0.co;2-1.

Differing degree and distribution of gastritis in Helicobacter pylori-associated diseases.

Virchows Arch. 1997 Jul;431(1):11-5. doi: 10.1007/s004280050063.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

α-连环蛋白在人类胃癌及一级亲属的胃黏膜中表达降低。

alpha-catenin expression is decreased in human gastric cancers and in the gastric mucosa of first degree relatives.

作者信息

Yu J, Ebert M P, Miehlke S, Rost H, Lendeckel U, Leodolter A, Stolte M, Bayerdörffer E, Malfertheiner P

机构信息

Department of Gastroenterology, Hepatology, and Infectious Diseases, Otto-von-Guericke University, D-39120 Magdeburg, Germany.

出版信息

Gut. 2000 May;46(5):639-44. doi: 10.1136/gut.46.5.639.

DOI:10.1136/gut.46.5.639

PMID:10764706

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1727909/

Abstract

BACKGROUND AND AIMS

METHODS

RESULTS

CONCLUSION

Our findings suggest that loss or downregulation of alpha-catenin expression may be an early event in gastric carcinogenesis and may be associated with H pylori infection.

摘要

背景与目的

方法

结果

结论

我们的研究结果表明，α-连环蛋白表达的缺失或下调可能是胃癌发生的早期事件，且可能与幽门螺杆菌感染有关。

α-连环蛋白在人类胃癌及一级亲属的胃黏膜中表达降低。

alpha-catenin expression is decreased in human gastric cancers and in the gastric mucosa of first degree relatives.

作者信息

机构信息

出版信息

BACKGROUND AND AIMS

METHODS

RESULTS

CONCLUSION

背景与目的

方法

结果

结论

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

α-连环蛋白在人类胃癌及一级亲属的胃黏膜中表达降低。

alpha-catenin expression is decreased in human gastric cancers and in the gastric mucosa of first degree relatives.

作者信息

机构信息

出版信息

BACKGROUND AND AIMS

METHODS

RESULTS

CONCLUSION

背景与目的

方法

结果

结论