Quintela-Sabarís Celestino, Kidd Petra S, Fraga María Isabel
Department of Botany, University of Santiago de Compostela, Spain.
Z Naturforsch C J Biosci. 2005 Mar-Apr;60(3-4):229-35. doi: 10.1515/znc-2005-3-408.
The genetic diversity of Cistus ladanifer ssp. ladanifer (Cistaceae) growing on ultramafic and non-ultramafic (basic and schists) soils in the NE of Portugal was studied in order to identify molecular markers that could distinguish the metal-tolerant ecotypes of this species. Random Amplified Polymorphic DNA (RAPD) markers were used in order to estimate genetic variation and differences between populations. The RAPD dataset was analysed by means of a cluster analysis and an analysis of molecular variance (AMOVA). Our results indicate a significant partitioning of molecular variance between ultramafic and non-ultramafic populations of Cistus ladanifer, although the highest percentage of this variance was found at the intra-population level. Mantel's test showed no relationship between inter-population genetic and geographic distances. A series of RAPD bands that could be related to heavy metal tolerance were observed. The identification of such markers will enable the use of Cistus ladanifer in phytoremediation procedures.
为了鉴定能够区分该物种耐金属生态型的分子标记，对生长在葡萄牙东北部超镁铁质和非超镁铁质（基性岩和片岩）土壤上的岩蔷薇（Cistus ladanifer ssp. ladanifer，半日花科）的遗传多样性进行了研究。使用随机扩增多态性DNA（RAPD）标记来估计种群间的遗传变异和差异。通过聚类分析和分子方差分析（AMOVA）对RAPD数据集进行分析。我们的结果表明，岩蔷薇超镁铁质和非超镁铁质种群之间的分子方差存在显著划分，尽管该方差的最高百分比出现在种群内水平。曼特尔检验表明种群间遗传距离与地理距离之间没有关系。观察到一系列可能与重金属耐受性相关的RAPD条带。鉴定出此类标记将有助于在植物修复过程中使用岩蔷薇。
