Defrère Sylvie, Van Langendonckt Anne, Moulin Pierre, Befahy Philippe, Gonzalez Dolores, Martinez-Madrid Belen, Dolmans Marie-Madeleine, Donnez Jacques
Department of Gynaecology, Université Catholique de Louvain, Brussels, Belgium.
Am J Reprod Immunol. 2005 Jul;54(1):5-12. doi: 10.1111/j.1600-0897.2005.00272.x.
Intercellular adhesion molecule-1 (ICAM-1) is thought to play an important role in pathophysiological processes in endometrial tissue. The aim of this study was to quantify and compare the expression of ICAM-1 mRNA and protein in cultured endometrial epithelial cells (EEC) versus endometrial stromal cells (ESC).
EEC and ESC were isolated from human endometrium and cultured. At confluency, ICAM-1 mRNA was measured by real-time reverse transcriptase-polymerase chain reaction, the membrane-bound form (mICAM-1) by immunocytodensitometry, and the soluble form (sICAM-1) by enzyme-linked immunosorbent assay.
At the transcriptional level, we observed a 1.7-fold increase in ICAM-1 expression in EEC versus ESC. mICAM-1 immunostaining in EEC [cell-relative membrane-bound signal-specific optical density (CR-M-SOD): 0.056 +/- 0.05] was stronger (P < 0.05) than in ESC (CR-M-SOD: 0.009 +/- 0.07). EEC were found to secrete more (P < 0.01) sICAM-1 ([sICAM-1] = 15.59 +/- 2.96 ng/mL) than ESC ([sICAM-1] = 5.14 +/- 2.61 ng/mL).
Cultured EEC constitutively express significantly more ICAM-1 mRNA and protein than ESC.
细胞间黏附分子-1（ICAM-1）被认为在子宫内膜组织的病理生理过程中起重要作用。本研究的目的是定量并比较培养的子宫内膜上皮细胞（EEC）与子宫内膜基质细胞（ESC）中ICAM-1 mRNA和蛋白的表达。
从人子宫内膜分离并培养EEC和ESC。汇合时，通过实时逆转录聚合酶链反应测量ICAM-1 mRNA，通过免疫细胞密度测定法测量膜结合形式（mICAM-1），通过酶联免疫吸附测定法测量可溶性形式（sICAM-1）。
在转录水平上，我们观察到EEC中ICAM-1的表达比ESC增加了1.7倍。EEC中的mICAM-1免疫染色[细胞相对膜结合信号特异性光密度（CR-M-SOD）：0.056±0.05]比ESC中的更强（P<0.05）（CR-M-SOD：0.009±0.07）。发现EEC分泌的sICAM-1更多（P<0.01）（[sICAM-1]=15.59±2.96 ng/mL），而ESC分泌的sICAM-1为（[sICAM-1]=5.14±2.61 ng/mL）。
培养的EEC组成性表达的ICAM-1 mRNA和蛋白明显多于ESC。
