Vujisić Sanja, Lepej Snjezana Zidovec, Jerković Lidija, Emedi Ivana, Sokolić Bozica
Department of Obstetrics and Gynecology, Medical School, University of Zagreb, Sveti Duh Hospital, Zagreb.
Am J Reprod Immunol. 2005 Jul;54(1):13-20. doi: 10.1111/j.1600-0897.2005.00274.x.
Data given in the literature, regarding the influence of antisperm antibodies (ASA) in the semen and/or sera on in vitro fertilization (IVF) procedure outcome are controversial. The aim of this study was to compare the prevalence of ASA in semen, peripheral blood and follicular fluid as well as to determine total immunoglobulin concentration in the serum and follicular fluid. Selected parameters were analyzed with regard to IVF outcome.
The study enrolled 52 married couples. ASA in the semen was determined by direct immunobead mixed antiglobulin reaction (MAR Screen test), while in the peripheral blood and follicular fluid was determined by indirect immunobead MAR Screen test. Immunoglobulin (IgG, IgM and IgA) concentration in the follicular fluid and serum was determined by a liquid-phase immunoprecipitation assay with nephelometric end-point detection and analyzed with regard to IVF outcome.
Semen MAR test IgG was < 20% in 38 couples, and > 20% in 14 couples. Fertilization (73.2% versus 71.5%) and pregnancy rates (28.9% versus 28.57%) in both groups of patients were not significantly different. The results of direct and indirect MAR test were not associated with fertilization and pregnancy rates. Total serum IgG, IgM and IgA in infertile women were within normal ranges. Follicular fluid IgG was within normal values for serum samples, while IgA and IgM were decreased.
The presence of ASA on sperm or in the serum and follicular fluid was not associated with IVF outcome in the couples with good quality semen characteristic.
文献中给出的关于精液和/或血清中的抗精子抗体（ASA）对体外受精（IVF）程序结果影响的数据存在争议。本研究的目的是比较精液、外周血和卵泡液中ASA的患病率，并测定血清和卵泡液中的总免疫球蛋白浓度。针对IVF结果分析选定的参数。
该研究纳入了52对已婚夫妇。精液中的ASA通过直接免疫珠混合抗球蛋白反应（MAR筛查试验）测定，而外周血和卵泡液中的ASA通过间接免疫珠MAR筛查试验测定。卵泡液和血清中的免疫球蛋白（IgG、IgM和IgA）浓度通过具有比浊终点检测的液相免疫沉淀测定法测定，并针对IVF结果进行分析。
38对夫妇的精液MAR试验IgG<20%，14对夫妇的>20%。两组患者的受精率（73.2%对71.5%）和妊娠率（28.9%对28.57%）无显著差异。直接和间接MAR试验结果与受精率和妊娠率无关。不孕女性血清中的总IgG、IgM和IgA在正常范围内。卵泡液IgG在血清样本的正常范围内，而IgA和IgM降低。
在精液质量良好的夫妇中，精子上或血清及卵泡液中ASA的存在与IVF结果无关。
