双管法和游泳法处理后的精子参数、ASA 和细胞凋亡。
Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods.
机构信息
Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Key Laboratory for Improving Birth Outcome Technique, Maternal and Child Health Care Hospital of Shandong Province, Jinan, Shandong, People's Republic of China.
Shandong Provincial Institute of Control of Endemic Disease, Jinan, Shandong, People's Republic of China.
出版信息
Am J Mens Health. 2021 Mar-Apr;15(2):15579883211001202. doi: 10.1177/15579883211001202.
The aim of this study was to improve the quality of semen samples by using a novel double-tube (DT) method. The DT method was developed to select sperm and compared with traditional swim-up (SU) technique for 31 semen samples. Sperm DNA integrity were tested with TUNEL and SCSA. Content of antisperm antibodies (ASA) in the semen was measured by ELISA and MAR. Levels of the caspase-3 in the sperm were assessed by western blotting. After SU and DT, 15 couples and 16 couples were underwent IVF-ET. The number of RCDs, the percentage of SDF and DFI, ASA and the level of caspase-3 were significantly decreased after DT and SU ( = .001 and .001). When the DT and SU compared, there were significant changes in the number of RCD, the percentage of SDF and DFI, ASA and the level of caspase-3 ( 0.05-0.001). There was a higher cleavage rate ( = .017) and a lower abortion rate ( .05) in DT-IVF group than in SU-IVF group. DT selection yielded spermatozoa with low RCDs, DFI, ASA, and caspase-3 which would be benefit for ART.
本研究旨在通过使用新型双管（DT）方法提高精液样本的质量。DT 方法是为了选择精子而开发的，并与传统的泳动（SU）技术对 31 个精液样本进行了比较。使用 TUNEL 和 SCSA 测试精子 DNA 完整性。通过 ELISA 和 MAR 测量精液中的抗精子抗体（ASA）含量。通过 Western blot 评估精子中的 caspase-3 水平。SU 和 DT 后，15 对夫妇和 16 对夫妇接受了 IVF-ET。DT 和 SU 后，RCD 数量、SDF 和 DFI 的百分比、ASA 和 caspase-3 水平均显著降低（=.001 和 .001）。当 DT 和 SU 比较时，RCD 数量、SDF 和 DFI 的百分比、ASA 和 caspase-3 水平均有显著变化（0.05-0.001）。DT-IVF 组的卵裂率较高（=.017），流产率较低（.05）。DT 选择产生了具有低 RCD、DFI、ASA 和 caspase-3 的精子，这将有利于 ART。
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