角膜移植排斥反应患者房水中的α-1抗胰蛋白酶
Alpha-1--antitrypsin in aqueous humour from patients with corneal allograft rejection.
作者信息
Funding Mikkel, Vorum Henrik, Nexø Ebba, Ehlers Niels
机构信息
Department of Ophthalmology, Arhus University Hospital, Arhus, Denmark.
出版信息
Acta Ophthalmol Scand. 2005 Jun;83(3):379-84. doi: 10.1111/j.1600-0420.2005.00457.x.
PURPOSE
To investigate the presence and concentration of alpha1-antitrypsin in aqueous humour at the time of corneal rejection and to compare results obtained from patients with reversible and irreversible rejection.
METHODS
Samples of aqueous humour were obtained from 17 patients with acute corneal endothelial allograft rejection. The presence of alpha1-antitrypsin in aqueous humour was confirmed by immunoblotting and measured employing a sandwich ELISA. Total protein concentrations in aqueous humour were measured using Bradford's method. The outcome of corneal rejection episodes was determined 1 month after diagnosing corneal rejection and described as reversible or irreversible rejection.
RESULTS
alpha1-antitrypsin was detected in aqueous humour. Patients with reversible rejection had significantly higher alpha1-antitrypsin concentration than patients with irreversible rejection (p = 0.044). There was no significant difference in total protein concentrations (p = 0.745), and no correlation was found between alpha1-antitrypsin and total protein concentrations (p = 0.368).
CONCLUSIONS
alpha1-antitrypsin in aqueous humour seems to signal a favourable outcome of corneal rejection. The possible mechanism is discussed.
目的
研究角膜排斥反应发生时房水中α1-抗胰蛋白酶的存在情况及浓度，并比较可逆性和不可逆性排斥反应患者的检测结果。
方法
从17例急性角膜内皮同种异体移植排斥反应患者中获取房水样本。通过免疫印迹法确认房水中α1-抗胰蛋白酶的存在，并采用夹心酶联免疫吸附测定法进行测量。使用Bradford法测量房水中的总蛋白浓度。在诊断角膜排斥反应1个月后确定角膜排斥反应的结果，并描述为可逆性或不可逆性排斥反应。
结果
在房水中检测到α1-抗胰蛋白酶。可逆性排斥反应患者的α1-抗胰蛋白酶浓度显著高于不可逆性排斥反应患者（p = 0.044）。总蛋白浓度无显著差异（p = 0.745），且未发现α1-抗胰蛋白酶与总蛋白浓度之间存在相关性（p = 0.368）。
结论
房水中的α1-抗胰蛋白酶似乎预示着角膜排斥反应的良好结果。对可能的机制进行了讨论。
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