Vural Perin, Akgül Cemil, Canbaz Mukaddes
Department of Biochemistry and Clinical Biochemistry, Istanbul Medical Facility, Capa 34093, Istanbul, Turkey.
Ann Clin Biochem. 2005 May;42(Pt 3):220-3. doi: 10.1258/0004563053857941.
Oxidative stress has been implicated in the pathogenesis of ageing and menopause, and can arise through the increased production of lipid peroxides and/or a deficiency of antioxidant defence.
To investigate the effects of the menopause and tibolone treatment (2.5 mg/day for six months) on plasma antioxidants and lipid peroxidation.
Plasma concentrations of ascorbic acid, alpha-tocopherol, total thiol groups, caeruloplasmin, erythrocyte glutathione (GSH) and malondialdehyde (MDA) were measured in 24 postmenopausal and 24 premenopausal healthy women.
Data analysis indicates a significant decrease in plasma ascorbic acid, alpha-tocopherol, total thiol groups, [corrected]erythrocyte GSH and a significant increase in lipid peroxides (expressed as MDA concentrations) in postmenopausal women. There was no significant difference between control and study groups in the mean plasma caeruloplasmin concentrations. It was found that there is a significant increase in alpha-tocopherol and significant decrease in lipid peroxide concentrations in postmenopausal after tibolone treatment.
The menopause is associated with an increase in oxidative stress and a decrease of some antioxidants, such as ascorbic acid, alpha-tocopherol, total thiols and erythrocyte GSH. Tibolone treatment leads to a decrease in concentrations of plasma lipid peroxide, probably by stimulating direct and indirect mechanisms of tocopherol regeneration and increasing plasma concentrations of vitamin E. However, due to the relatively small numbers involved this study can be regarded as a pilot. Further studies performed on a larger scale are necessary to establish the exact mechanisms of tibolone in inhibiting oxidative stress in postmenopausal women.
氧化应激与衰老和绝经的发病机制有关，可通过脂质过氧化物生成增加和/或抗氧化防御不足而产生。
研究绝经及替勃龙治疗（2.5毫克/天，持续6个月）对血浆抗氧化剂和脂质过氧化的影响。
测定了24名绝经后和24名绝经前健康女性血浆中抗坏血酸、α-生育酚、总巯基、铜蓝蛋白、红细胞谷胱甘肽（GSH）和丙二醛（MDA）的浓度。
数据分析表明，绝经后女性血浆抗坏血酸、α-生育酚、总巯基、[校正后]红细胞GSH显著降低，脂质过氧化物（以MDA浓度表示）显著增加。对照组和研究组血浆铜蓝蛋白平均浓度无显著差异。发现替勃龙治疗后绝经后女性α-生育酚显著增加，脂质过氧化物浓度显著降低。
绝经与氧化应激增加及某些抗氧化剂减少有关，如抗坏血酸、α-生育酚、总巯基和红细胞GSH。替勃龙治疗可能通过刺激生育酚再生的直接和间接机制并增加血浆维生素E浓度，导致血浆脂质过氧化物浓度降低。然而，由于参与研究的人数相对较少，本研究可视为一项初步研究。有必要进行更大规模的进一步研究，以确定替勃龙抑制绝经后女性氧化应激的确切机制。
