[评价分枝杆菌散布重复单位分型作为结核分枝杆菌分子流行病学实用方法的研究]

[Evaluation of the mycobacterial interspersed repetitive units typing as a practical approach in molecular epidemiology of Mycobacterium tuberculosis].

作者信息

Shen Guo-miao, Zha Jia, Xu Lin, Sun Bin, Gui Xiao-hong, Wang Yi-feng, Mei Jian, Gao Qian

机构信息

Key Laboratory of Medical Molecular Virology of Ministry of Education, Shanghai Medical School, Fudan University, Shanghai 200032, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2005 May;28(5):292-6.

PMID:15949306

Abstract

OBJECTIVE

To introduce a new genotyping method, mycobacterial interspersed repetitive units (MIRU) typing, for Mycobacterium tuberculosis and to evaluate its feasibility.

METHODS

Mycobacterium tuberculosis strains stored at Shanghai Municipal Center for Disease Control and Prevention during 2000 to 2002, were randomly selected by simple digital table and genotyped by MIRU and Spoligotyping.

RESULTS

By Spoligotyping method, 91 strains were typed to 20 genotypes, of which 89% (81/91) strains belonged to Beijing genotype, while by MIRU method, these strains were divided into 46 genotypes. The MIRU typing showed high discriminatory power, especially for the Beijing genotype strains. The 81 Beijing genotype strains could be subdivided into 39 different MIRU genotypes. In this sample collection, 12 MIRU loci showed different discriminative according to their allelic diversity. Locus 26 showed highly discriminative, while locus 16, 31, and 40 showed moderately discriminative.

CONCLUSIONS

MIRU genotyping is a simple and fast method. Its numerical result facilitates the comparison among strains of Mycobacterium tuberculosis from different labs.

摘要

目的

介绍一种用于结核分枝杆菌的新基因分型方法——分枝杆菌散布重复单位（MIRU）分型，并评估其可行性。

方法

采用简单数字表法从上海市疾病预防控制中心2000年至2002年保存的结核分枝杆菌菌株中随机选取菌株，采用MIRU和间隔寡核苷酸分型法（Spoligotyping）进行基因分型。

结果

采用Spoligotyping法，91株菌株被分为20种基因型，其中89%（81/91）的菌株属于北京基因型；而采用MIRU法，这些菌株被分为46种基因型。MIRU分型显示出较高的鉴别力，尤其是对于北京基因型菌株。81株北京基因型菌株可进一步细分为39种不同的MIRU基因型。在该样本收集中，12个MIRU位点根据其等位基因多样性显示出不同的鉴别力。位点26显示出高鉴别力，而位点16、31和40显示出中等鉴别力。