Rima B K, Curran M D, Kennedy S
Division of Genetic Engineering, School of Biology and Biochemistry, Queen's University of Belfast, UK.
Sci Total Environ. 1992 Apr 20;115(1-2):45-55. doi: 10.1016/0048-9697(92)90031-m.
The biochemical characterisation of phocine distemper virus (PDV) has shown that PDV is related to but clearly distinct from canine distemper virus (CDV) and relative to its relationship with CDV is only remotely related to the other morbilliviruses, namely measles virus (MV) or rinderpest virus (RPV) and peste-des-petits-ruminants virus (PPRV). Comparative studies with monoclonal antibodies indicate that the virus is serologically closely related to CDV with many conserved epitopes, particularly on the internal proteins of the virus, while the external attachment (H) protein shows the greatest level of variability among the distemper virus isolates. The analysis of the viral proteins by electrophoresis indicates molecular weight differences between CDV and PDV in the fusion (F), phosphoprotein (P), H, nucleocapsid (N) and matrix (M) proteins. The RNA profiles of CDV and PDV are indistinguishable and different from those for RPV and MV. Nucleotide sequence analysis of cDNA clones of the virus show approximately 70% homology between CDV and PDV and approximately 48% with MV. These data prove that PDV is a different virus from CDV and co-circulates with it probably primarily in sea mammals.
海豹瘟热病毒（PDV）的生化特性表明，PDV与犬瘟热病毒（CDV）相关，但明显不同，并且相对于其与CDV的关系，它与其他麻疹病毒，即麻疹病毒（MV）、牛瘟病毒（RPV）和小反刍兽疫病毒（PPRV）的关系非常疏远。与单克隆抗体的比较研究表明，该病毒在血清学上与CDV密切相关，具有许多保守表位，特别是在病毒的内部蛋白上，而外部附着（H）蛋白在瘟热病毒分离株中显示出最大程度的变异性。通过电泳对病毒蛋白进行分析表明，CDV和PDV在融合（F）蛋白、磷蛋白（P）、H蛋白、核衣壳（N）蛋白和基质（M）蛋白上存在分子量差异。CDV和PDV的RNA图谱无法区分，且与RPV和MV的RNA图谱不同。对该病毒cDNA克隆的核苷酸序列分析表明，CDV和PDV之间的同源性约为70%，与MV的同源性约为48%。这些数据证明，PDV是一种与CDV不同的病毒，可能主要在海洋哺乳动物中与CDV共同传播。
