Law Mark E, Templeton Kristen L, Kitange Gaspar, Smith Justin, Misra Anjan, Feuerstein Burt G, Jenkins Robert B
Department of Laboratory Medicine and Pathology, Division of Laboratory Genetics, Mayo Clinic and Foundation, 200 First Street SW, Rochester, MN 55905, USA.
Cancer Genet Cytogenet. 2005 Jul 1;160(1):1-14. doi: 10.1016/j.cancergencyto.2004.11.012.
Deletions of chromosome 1p and 19q arms are frequent genetic abnormalities in primary human gliomas and are especially common in oligodendrogliomas. However, the chromosome 1p and 19q status of many glioma cell lines has not been established. Using homozygosity mapping, fluorescence in situ hybridization (FISH), and comparative genomic hybridization to arrayed BAC (CGHa), we screened 17 glioma cell lines for chromosome 1 and 19 deletions. Sequence tagged site polymorphisms were used to evaluate the cell lines for regions of chromosome 1p and 19q homozygosity. Cell lines A172, U251, TP265, U118, SW1088, U87, SW1783, and D32 contained significant regions of 19q homozygosity. In addition, A172, U87, TP483, D37, U118, MO67, and TP265 contained significant regions of 1p homozygosity. FISH probes localized to 1p36.32 and 19q13.33 as well as CGHa were used to determine which cell lines had deletions of 1p and/or 19q. Cell lines A172, U87, TP483, TP265, H4, U251, and D37 were deleted for portions of 1p. CGHa and homozygosity mapping of these cell lines define a 700-kilobase (Kb) common deletion region that is encompassed by a larger deletion region previously mapped in sporadic gliomas. This common deletion region is localized at 1p36.31 and includes CHD5, a putative tumor suppressor gene. Cell line A172 was observed to have a deletion between 19q13.33 and 19q13.41, while U87 was observed to have a smaller deletion of 19q13.33. Cell lines A172 and U87 contain 1p and 19q deletions similar to those found in sporadic gliomas and will be useful cellular reagents for evaluating the function of putative 1p and 19q glioma tumor suppressor genes.
1p和19q臂的缺失是原发性人类胶质瘤中常见的基因异常，在少突胶质细胞瘤中尤为常见。然而，许多胶质瘤细胞系的1p和19q染色体状态尚未明确。我们使用纯合性定位、荧光原位杂交（FISH）以及与BAC阵列的比较基因组杂交（CGHa），对17个胶质瘤细胞系进行了1号和19号染色体缺失的筛查。序列标签位点多态性用于评估细胞系1p和19q染色体的纯合区域。细胞系A172、U251、TP265、U118、SW1088、U87、SW1783和D32含有19q染色体的显著纯合区域。此外，A172、U87、TP483、D37、U118、MO67和TP265含有1p染色体的显著纯合区域。定位到1p36.32和19q13.33的FISH探针以及CGHa被用于确定哪些细胞系存在1p和/或19q的缺失。细胞系A172、U87、TP483、TP265、H4、U251和D37的1p部分存在缺失。这些细胞系CGHa和纯合性定位确定了一个700千碱基（Kb）的共同缺失区域，该区域被先前在散发性胶质瘤中定位的一个更大的缺失区域所包含。这个共同缺失区域位于1p36.31，包括一个假定的肿瘤抑制基因CHD5。观察到细胞系A172在19q13.33和19q13.41之间存在缺失，而U87在19q13.33存在较小的缺失。细胞系A172和U87含有与散发性胶质瘤中发现的类似的1p和19q缺失，将成为评估假定的1p和19q胶质瘤肿瘤抑制基因功能的有用细胞试剂。
