一种用于同时靶向抗原的多色荧光免疫染色技术。

A multicolor fluorescence immunostaining technique for simultaneous antigen targeting.

作者信息

Buchwalow Igor B, Minin Evgeny A, Boecker Werner

机构信息

Interdisciplinary Center of Clinical Research, Gerhard Domagk Institute of Pathology, University of Muenster, Domagkstr. 17, D-48149 Muenster, Germany.

出版信息

Acta Histochem. 2005;107(2):143-8. doi: 10.1016/j.acthis.2005.01.003. Epub 2005 Apr 1.

DOI:10.1016/j.acthis.2005.01.003

PMID:15950054

Abstract

A general problem in immunocytochemistry is the development of a reliable multiple immunolabeling method with primary antibodies originating from the same host species. Here, we briefly outline different approaches intended to close this technological gap and focus on multiple immunolabeling with monoclonal primary antibodies. To this end, we generated a basic universal protocol for the use of secondary antibodies selectively recognizing different isotypes/subclasses of monoclonal primary antibodies. This approach is widely applicable and offers a simple procedure for simultaneously detecting two or more antigens.

摘要

免疫细胞化学中的一个普遍问题是开发一种可靠的多重免疫标记方法，该方法使用源自同一宿主物种的一抗。在此，我们简要概述旨在弥合这一技术差距的不同方法，并重点介绍使用单克隆一抗的多重免疫标记。为此，我们生成了一个基本通用方案，用于使用选择性识别单克隆一抗不同同种型/亚类的二抗。这种方法具有广泛的适用性，并提供了一种同时检测两种或更多种抗原的简单程序。