Lee Sang-Nam, Choi Jin-Ha, Cho Hyeon-Yeol, Choi Jeong-Woo
Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-Gu, Seoul 04107, Korea.
Uniance Gene Inc., 1107 Teilhard Hall, 35 Baekbeom-Ro, Mapo-Gu, Seoul 04107, Korea.
Pharmaceutics. 2020 Jan 7;12(1):50. doi: 10.3390/pharmaceutics12010050.
The biosensing platform is noteworthy for high sensitivity and precise detection of target analytes, which are related to the status of cells or specific diseases. The modification of the transducers with metallic nanoparticles (MNPs) has attracted attention owing to excellent features such as improved sensitivity and selectivity. Moreover, the incorporation of MNPs into biosensing systems may increase the speed and the capability of the biosensors. In this review, we introduce the current progress of the developed cell-based biosensors, cell chip, based on the unique physiochemical features of MNPs. Mainly, we focus on optical intra/extracellular biosensing methods, including fluorescence, localized surface plasmon resonance (LSPR), and surface-enhanced Raman spectroscopy (SERS) based on the coupling of MNPs. We believe that the topics discussed here are useful and able to provide a guideline in the development of new MNP-based cell chip platforms for pharmaceutical applications such as drug screening and toxicological tests in the near future.
生物传感平台因其对与细胞状态或特定疾病相关的目标分析物具有高灵敏度和精确检测能力而备受关注。用金属纳米颗粒（MNP）修饰换能器因其具有诸如提高灵敏度和选择性等优异特性而受到关注。此外，将MNP纳入生物传感系统可能会提高生物传感器的速度和能力。在本综述中，我们基于MNP独特的物理化学特性，介绍了已开发的基于细胞的生物传感器即细胞芯片的当前进展。主要地，我们关注基于MNP耦合的光学细胞内/外生物传感方法，包括荧光、局域表面等离子体共振（LSPR）和表面增强拉曼光谱（SERS）。我们相信，这里讨论的主题是有用的，并且能够为在不久的将来开发用于药物应用（如药物筛选和毒理学测试）的新型基于MNP的细胞芯片平台提供指导。
