Raudenkolb Steve, Wartewig Siegfried, Brezesinski Gerald, Funari Sergio S, Neubert Reinhard H H
Department of Pharmacy, Martin-Luther-University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, D-06120 Halle/Saale, Germany.
Chem Phys Lipids. 2005 Jul;136(1):13-22. doi: 10.1016/j.chemphyslip.2005.03.005.
The thermotropic properties of N-(alpha-hydroxyacyl)-sphingosine (CER[AS]) in dry and hydrated state were studied by means of X-ray powder diffraction and FT-Raman spectroscopy. The polymorphic states of the CER[AS]/water mixture (lamellar crystalline, lamellar hexagonal gel, liquid crystalline) depend on the thermal pre-treatment of the sample. Only by heating the CER[AS]/water mixture above the melting chain transition can the system be hydrated. At room temperature, both dry and hydrated states form lamellar structures, which differ in their repeat distance and packing of hydrocarbon chains. Above the melting chain transition, hydrated CER[AS] forms a liquid crystalline hexagonal phase, whereas anhydrous CER[AS] forms an isotropic liquid phase. The various phases of hydrated CER[AS] are distinguished on the basis of the corresponding Raman spectra.
通过X射线粉末衍射和傅里叶变换拉曼光谱研究了N-(α-羟基酰基)-鞘氨醇（CER[AS]）在干燥和水合状态下的热致性质。CER[AS]/水混合物的多晶态（层状晶体、层状六方凝胶、液晶）取决于样品的热预处理。只有将CER[AS]/水混合物加热到熔点链转变温度以上，体系才能水合。在室温下，干燥和水合状态均形成层状结构，其重复距离和烃链堆积不同。在熔点链转变温度以上，水合CER[AS]形成液晶六方相，而无水CER[AS]形成各向同性液相。根据相应的拉曼光谱区分水合CER[AS]的不同相。
