Eilts Bruce E
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Theriogenology. 2005 Aug;64(3):685-91. doi: 10.1016/j.theriogenology.2005.05.017.
Evaluation of canine cryopreserved semen has the ultimate goal of determining if an individual frozen ejaculate will have acceptable fertility. This is difficult in that there is no accepted normal fertility for the dog. The fertility of the female also plays a crucial role in estimating the fertility of the male. Poor female fertility can make a fertile male appear less fertile. Variability of animals, breeding technique, breeding timing, and number of cells inseminated make comparisons in canine fertility difficult to truly measure. Many more animals are needed to provide meaningful statistical results than are usually used. Several tests, including motility in bright field and phase contrast microscopy, computer analysis of motility, sperm morphology, sperm membrane integrity, capacitation and sperm function tests have been investigated to predict fertility, however few of these tests have actually been correlated with fertility. More work is needed to create one or more tests that accurately predict fertility of cryopreserved canine semen.
对犬冷冻精液进行评估的最终目标是确定个体冷冻射精精液是否具有可接受的生育能力。这很困难，因为犬没有公认的正常生育能力标准。雌性的生育能力在评估雄性生育能力方面也起着关键作用。雌性生育能力差会使有生育能力的雄性显得生育能力较低。动物的变异性、繁殖技术、繁殖时间以及输精的细胞数量使得犬类生育能力的比较难以真正衡量。要提供有意义的统计结果，所需的动物数量比通常使用的要多得多。已经研究了几种测试，包括明视野和相差显微镜下的活力、活力的计算机分析、精子形态、精子膜完整性、获能和精子功能测试，以预测生育能力，然而这些测试中很少有与生育能力实际相关的。需要开展更多工作来创建一个或多个能够准确预测冷冻犬精液生育能力的测试。
