Blesbois E, Grasseau I, Seigneurin F, Mignon-Grasteau S, Saint Jalme M, Mialon-Richard M M
INRA, UMR-CNRS-Haras Nationaux-Université de Tours 0085, F-37380, Nouzilly, France.
Theriogenology. 2008 Jan 15;69(2):252-61. doi: 10.1016/j.theriogenology.2007.09.019. Epub 2007 Oct 30.
Semen cryopreservation is very important for the ex situ management of genetic diversity in birds but it is rarely used. This is partly because of the highly variable success rates, and this emphasizes the need for predictors of semen freezability. This study evaluated the ability of semen quality tests to predict the success rates of semen cryopreservation in chickens and the relationships between each test. Individual variations of in vitro quality tests of semen were compared to the fertility obtained with fresh and cryopreserved semen. The in vitro semen quality tests represented viability, integrity, motility (percentage of viable and morphologically normal cells (PVN); mass motility (MMOT) and different motion parameters including percentage of motile spermatozoa (PMOT)) and biophysical tests (OSM, resistance to osmotic stress; membrane fluidity (FLUID)). Different in vitro tests were significantly correlated between each other for fresh (MMOT, PVN and FLUID, many criteria of objective motility) and cryopreserved semen (MMOT, different objective motility parameters, PVN). Fertility was significantly correlated with PVN for fresh semen and PVN and different objective motility criteria for cryopreserved semen. Membrane fluidity, followed by PVN, PMOT and MMOT, measured on fresh semen samples was positively correlated with fertility obtained with cryopreserved semen. The combination of the first three tests explained 85% of the variability of fertility observed with cryopreserved semen. In conclusion, we showed that different in vitro tests of semen quality are of predictive value for the success rate of semen cryopreservation in the chicken, the most accurate being membrane fluidity.
精液冷冻保存对于鸟类遗传多样性的迁地管理非常重要，但很少被使用。部分原因是成功率高度可变，这凸显了精液冷冻能力预测指标的必要性。本研究评估了精液质量检测预测鸡精液冷冻保存成功率的能力以及各检测之间的关系。将精液体外质量检测的个体差异与新鲜精液和冷冻精液的受精率进行比较。体外精液质量检测包括活力、完整性、运动性（活的和形态正常细胞的百分比（PVN）；总运动性（MMOT）以及不同的运动参数，包括活动精子百分比（PMOT））和生物物理检测（OSM，抗渗透应激能力；膜流动性（FLUID））。新鲜精液（MMOT、PVN和FLUID，许多客观运动性标准）和冷冻精液（MMOT、不同的客观运动性参数、PVN）的不同体外检测之间彼此显著相关。新鲜精液的受精率与PVN显著相关，冷冻精液的受精率与PVN和不同的客观运动性标准显著相关。新鲜精液样本上测得的膜流动性，其次是PVN、PMOT和MMOT，与冷冻精液获得的受精率呈正相关。前三项检测的组合解释了冷冻精液观察到的受精率变异性的85%。总之，我们表明精液质量的不同体外检测对鸡精液冷冻保存的成功率具有预测价值，最准确的是膜流动性。
