使用高效液相色谱法来证明泰国眼镜蛇（眼镜王蛇和暹罗眼镜蛇）毒液中毒素成分的变化。
Use of HPLC to demonstrate variation of venom toxin composition in the Thailand cobra venoms Naja naja kaouthia and Naja naja siamensis.
作者信息
Namiranian S, Hider R C
机构信息
Department of Pharmacy, King's College, University of London, U.K.
出版信息
Toxicon. 1992 Jan;30(1):47-61. doi: 10.1016/0041-0101(92)90501-u.
The composition of the venoms of Naja naja kaouthia and Naja naja siamensis from different commercial sources has been investigated using both ion-exchange and reverse-phase high-pressure liquid chromatography (RP-HPLC) in order to investigate variation in toxin contents. The venoms contained identical major toxin components, although in different relative concentrations. The venom collected separately from the left and right glands of individual snakes were virtually the same as judged by RP-HPLC. The cytotoxin CT-II, which was previously only reported to be present in Naja naja siamensis venom, was detected in all the venoms investigated. Two long neurotoxin homologues have also been isolated.
为了研究毒素含量的变化，使用离子交换和反相高压液相色谱法（RP-HPLC）对来自不同商业来源的眼镜王蛇和暹罗眼镜蛇的毒液成分进行了研究。这些毒液含有相同的主要毒素成分，尽管相对浓度不同。通过RP-HPLC判断，从个体蛇的左右腺体分别采集的毒液几乎相同。在所有研究的毒液中都检测到了细胞毒素CT-II，该毒素此前仅报道存在于暹罗眼镜蛇毒液中。还分离出了两种长链神经毒素同系物。
Zotero 插件