Semsey Szabolcs, Virnik Konstantin, Adhya Sankar
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 USA.
Trends Biochem Sci. 2005 Jun;30(6):334-41. doi: 10.1016/j.tibs.2005.04.009.
Nucleoprotein complexes comprising short DNA loops (150 base pairs or less) are involved in a wide variety of DNA transactions (e.g. transcription regulation, replication and recombination) in both prokaryotes and eukaryotes, and also can be useful in designing nanostructures. In these higher-order nucleoprotein complexes, proteins bound to spatially separated sites on a DNA interact with each other by looping out the relatively stiff intervening DNA. Recent technological developments have enabled determination of DNA trajectories in a few DNA-loop-containing regulatory complexes. Results show that, in a given system, a specific DNA trajectory is preferred over others.
包含短DNA环（150个碱基对或更少）的核蛋白复合物参与原核生物和真核生物中各种各样的DNA活动（如转录调控、复制和重组），并且在设计纳米结构方面也可能有用。在这些高阶核蛋白复合物中，与DNA上空间分离位点结合的蛋白质通过使相对僵硬的中间DNA成环而相互作用。最近的技术发展使得在一些含DNA环的调控复合物中确定DNA轨迹成为可能。结果表明，在给定系统中，特定的DNA轨迹比其他轨迹更受青睐。
