西鹃杂种的离体多倍体诱导

In vitro polyploidy induction in Rhododendron simsii-hybrids.

作者信息

Eeckhout T, Samyn G, Van Bockstaele E

机构信息

Department of Plant Genetics and Breeding, Caritasstaat 21, 9090 Melle, Belgium.

出版信息

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet. 2001;66(3b):451-4.

PMID:15954635

Abstract

Three in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was rather labour intensive. Direct sowing on media enriched with these compounds required less work but was less efficient. Mitotic inhibitors, when added to multiplication media, induced ploidy chimeras of existing genotypes.

摘要

评估了三种诱导四倍体西洋杜鹃杂种的体外培养方案。最成功的技术是每天对幼苗的顶端分生组织施用有丝分裂毒物。然而，这种技术相当耗费人力。直接播种在富含这些化合物的培养基上所需工作量较少，但效率较低。当将有丝分裂抑制剂添加到增殖培养基中时，会诱导现有基因型的倍性嵌合体。