Mo Lan, Chen Junhao, Lou Xiongzhen, Xu Qiangwei, Dong Renhui, Tong Zaikang, Huang Huahong, Lin Erpei
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China.
Plants (Basel). 2020 Mar 31;9(4):424. doi: 10.3390/plants9040424.
Polyploidy in has great potential to improve its horticultural and commercial value, and to also meet market demands. In this study, a feasible method for polyploid induction in via colchicine treatment was established, and the obtained polyploid plants were identified and characterized. As a result, the stem bases of tissue-cultured plantlets treated with 0.1% colchicine for 24 h showed the highest polyploid induction with a rate of 36.67%. By flow cytometric analysis, 69 tetraploids and 29 octoploids were identified in the regenerated plants that were examined. Phenotypic analysis indicated that the leaves of tetraploid and octoploid plants were smaller, rounder and thicker with more abundant and longer epidermal hairs than those of diploids. Furthermore, the stomata of polyploids were larger and sparser than those of diploids. An increase in chlorophyll content was also detected in polyploids, which resulted in darker green leaves. In conclusion, our study established an effective method to induce polyploidy in , which could be used to develop new genetic resources for breeding and other species in the future.
[植物名称]中的多倍体具有极大潜力来提升其园艺和商业价值，并满足市场需求。在本研究中，建立了一种通过秋水仙素处理诱导[植物名称]多倍体的可行方法，并对获得的多倍体植株进行了鉴定和特征分析。结果显示，用0.1%秋水仙素处理24小时的组培苗茎基部多倍体诱导率最高，为36.67%。通过流式细胞术分析，在检测的再生植株中鉴定出69株四倍体和29株八倍体。表型分析表明，四倍体和八倍体植株的叶片比二倍体植株的叶片更小、更圆、更厚，表皮毛更丰富且更长。此外，多倍体的气孔比二倍体的更大且更稀疏。多倍体中还检测到叶绿素含量增加，这导致叶片颜色更深绿。总之，我们的研究建立了一种在[植物名称]中诱导多倍体的有效方法，未来可用于开发[植物名称]及其他[植物名称]物种育种的新遗传资源。
