López-Camarillo César, Orozco Esther, Marchat Laurence A
Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo # 290, Col. del Valle, CP 03110, Mexico D.F., Mexico.
Exp Parasitol. 2005 Jul;110(3):184-90. doi: 10.1016/j.exppara.2005.02.024.
We report here the pre-mRNA 3' end processing machinery in Entamoeba histolytica. Comparative analysis of the putative sequences participating in the pre-mRNA 3' end processing of E. histolytica genes shows similitude and differences to those described for yeast and human transcripts. By a genomic survey, we identified 16 putative genes encoding for cleavage/polyadenylation factors in this parasite. E. histolytica pre-mRNA 3' end processing machinery does not seem to contain homologous genes coding for human Symplekin, CFIm59, and CFIm68 proteins, neither sequences related to yeast Pta1p and Hrp1p. Protein sequence comparisons among E. histolytica, yeast, and human showed little variation in their functional domains through evolutive scale. E. histolytica pre-mRNA 3' end processing machinery appears to be in an intermediate evolutionary position between mammals and yeast. From these analyses, we propose a hypothetical working model for the pre-mRNA 3' end processing machinery in E. histolytica.
我们在此报告溶组织内阿米巴的前体mRNA 3' 端加工机制。对参与溶组织内阿米巴基因前体mRNA 3' 端加工的假定序列进行的比较分析表明，其与酵母和人类转录本中描述的序列既有相似之处，也有不同之处。通过基因组调查，我们在这种寄生虫中鉴定出16个编码切割/聚腺苷酸化因子的假定基因。溶组织内阿米巴前体mRNA 3' 端加工机制似乎不包含编码人类Symplekin、CFIm59和CFIm68蛋白的同源基因，也没有与酵母Pta1p和Hrp1p相关的序列。溶组织内阿米巴、酵母和人类之间的蛋白质序列比较表明，在进化过程中其功能域变化不大。溶组织内阿米巴前体mRNA 3' 端加工机制似乎处于哺乳动物和酵母之间的中间进化位置。基于这些分析，我们提出了一个关于溶组织内阿米巴前体mRNA 3' 端加工机制的假设工作模型。
