Laboratorio de Ingeniería Genética y Biología Celular y Molecular-Área Virosis de Invertebrados, Departamento de Ciencia y Tecnología, Instituto de Microbiología Básica y Aplicada, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina.
Laboratorio de Virus Emergentes, Departamento de Ciencia y Tecnología, Instituto de Microbiología Básica y Aplicada, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina.
Viruses. 2020 Dec 6;12(12):1395. doi: 10.3390/v12121395.
Baculoviruses are a group of insect viruses with large circular dsDNA genomes exploited in numerous biotechnological applications, such as the biological control of agricultural pests, the expression of recombinant proteins or the gene delivery of therapeutic sequences in mammals, among others. Their genomes encode between 80 and 200 proteins, of which 38 are shared by all reported species. Thanks to multi-omic studies, there is remarkable information about the baculoviral proteome and the temporality in the virus gene expression. This allows some functional elements of the genome to be very well described, such as promoters and open reading frames. However, less information is available about the transcription termination signals and, consequently, there are still imprecisions about what are the limits of the transcriptional units present in the baculovirus genomes and how is the processing of the 3' end of viral mRNA. Regarding to this, in this review we provide an update about the characteristics of DNA signals involved in this process and we contribute to their correct prediction through an exhaustive analysis that involves bibliography information, data mining, RNA structure and a comprehensive study of the core gene 3' ends from 180 baculovirus genomes.
杆状病毒是一组具有大圆形 dsDNA 基因组的昆虫病毒，广泛应用于生物技术领域，如农业害虫的生物防治、重组蛋白的表达或治疗性序列在哺乳动物中的基因传递等。它们的基因组编码 80 到 200 种蛋白质，其中 38 种在所有报道的物种中都有共享。由于多组学研究，我们对杆状病毒的蛋白质组和病毒基因表达的时间性有了显著的了解。这使得基因组的一些功能元件，如启动子和开放阅读框，能够得到很好的描述。然而，关于转录终止信号的信息较少，因此，关于杆状病毒基因组中存在的转录单位的界限以及病毒 mRNA 3'端的加工方式，仍然存在一些不精确之处。关于这一点，在这篇综述中，我们提供了关于参与这个过程的 DNA 信号的特征的最新信息，并通过涉及文献信息、数据挖掘、RNA 结构和对 180 个杆状病毒基因组核心基因 3'端的综合研究的详尽分析，有助于对它们进行正确的预测。
